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Power SYBR® Green PCR Master Mix

Catalog number: 4368702

Applied Biosystems™  Related applications: Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling

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Description

Get superior nucleic acid quantitation with Applied Biosystems® Power SYBR® Green Master Mix. Power SYBR® Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.

• Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient single-tube format. Power SYBR® Green PCR Master Mix contains all of the components, excluding the template and primers, for superior SYBR® Green reagent-based real-time PCR
• The optimized formulation contains highly purified AmpliTaq Gold® DNA Polymerase, LD to offer greater sensitivity than classic SYBR® Green PCR master mix
Power SYBR® Green PCR Master Mix contains a blend of dTTP/dUTP that is compatible with AmpErase® UNG, to minimize carryover contamination
• Includes a proprietary version of ROX™ dye, an internal passive reference, to normalize non-PCR–related fluorescence fluctuations to minimize well-to-well variability that result from a variety of causes, such as pipetting error and sample evaporation
Power SYBR® Green reagent-based PCR chemistry easily replaces the existing SYBR® Green PCR Master Mix in Applied Biosystems® protocols using the same setup and thermal cycling conditions
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: SYBR
For Use With (Equipment): 7000 System, 7300 System, 7500 System, 7900HT System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): DNA
Label or Dye: SYBR® Green
Passive Reference Dye: ROX (Pre-mixed)
Polymerase: AmpliTaq Gold® DNA Polymerase
Primer-Probe Compatibility: Unlabeled Oligos
Product Size: 5 x 5 mL
Purity or Quality Grade: LD (Low DNA)
Sample Type (Specific): DNA (Genomic), cDNA

Contents & storage

The 2X mix contains SYBR® Green 1 Dye, AmpliTaq Gold® DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference 1, and optimized buffer components. Contains 1 × 50 mL bottle, sufficient for 1000 reactions of 50 µL each.

Store at 2–8°C.

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