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TaqMan® Genotyping Master Mix

Catalog number: 4371355

Applied Biosystems™  Related applications: Genotyping & Genomic Profiling | Pharma & Biopharma | Real Time PCR (qPCR) | Target & Lead Identification & Validation

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The Applied Biosystems® TaqMan® Genotyping Master Mix is optimized for end-point fluorescence detection in SNP genotyping applications.

• Distinct clusters and high call rates for unambiguous allelic discrimination
• Validated with TaqMan® SNP Genotyping Assays
• Excellent pre- and post-PCR stability for high throughput setup and analysis

May be used for a variety of genotyping applications, including:

• Candidate gene studies
• Drug-target validation
• Disease association studies
• Population genetics
• Linkage mapping
• Agricultural applications

Reaction size is based on a 50 µl reaction volume; please see below for other reaction sizes.

Exceptional Performance from an Optimized Mix (2X)
Accurate genotype assignments result from preferential binding of the allele-specific probe to the matching target. TaqMan® Genotyping Master Mix enables specific binding of the probe to achieve exceptional cluster resolution. Key features include:

• AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure) – Automatic hot start enzyme designed to be active during thermal cycling and inactive at room temperature for easy reaction setup.
• Optimized mix components provide excellent specificity for discrimination between alleles
• Passive internal reference based on proprietary ROX™ dye for increased precision on Applied Biosystems® real-time PCR instruments
• Single thermal cycling condition for consistent results with TaqMan® Assays

Validated with TaqMan® Genotyping Assays and Instruments
TaqMan® Genotyping Master Mix has been tested across all types of TaqMan® SNP Genotyping Assays: TaqMan® Drug Metabolism Genotyping Assays, TaqMan® SNP Genotyping Assays, and Custom TaqMan® SNP Genotyping Assays. In addition, the master mix is validated with the Applied Biosystems® thermal cyclers and real-time PCR systems.

High-Throughput Setup and Analysis
The combination of several low-cost thermal cyclers for PCR and a single real-time PCR instrument for allelic discrimination make high throughput SNP genotyping manageable. TaqMan® Genotyping Master Mix further improves high-throughput setup and analysis with excellent room temperature stability both before and after PCR. This provides the flexibility required to run long experiments, unattended overnight or over a weekend.

Reliable Discrimination with Challenging Targets
Amplicon design constraints make SNP detection challenging, particularly when targets contain an abundance of GC- or AT-rich regions. The optimized components of TaqMan® Genotyping Master Mix result in tight, well-separated clusters and more accurate allele-calls compared to other mixes, even for challenging targets.

Setting the Industry Standard for Quality
Extensive analytical and functional tests are performed for each manufactured lot of TaqMan® Genotyping Master Mix, including one TaqMan® SNP Genotyping Assay and one TaqMan® Drug Metabolism Genotyping Assay. Results are compiled in an informative Certificate of Analysis, which is accessible through the Applied Biosystems® Web site (search for 'Certificate of Analysis').

Number of Genotyping Reactions at a Given Reaction Size:

4371355, TaqMan® Genotyping Master Mix, 1-Pack (1 x 10 mL)
• 50 µL Reactions: 400
• 20 µL Reactions: 1000
• 10 µL Reactions: 2,000
• 5 µL Reactions: 4,000

4381656, TaqMan® Genotyping Master Mix, 2-Pack (2 x 10 mL)
• 50 µL Reactions: 800
• 20 µL Reactions: 2,000
• 10 µL Reactions: 4,000
• 5 µL Reactions: 8,000

4371357, TaqMan® Genotyping Master Mix, (1 x 50 mL)
• 50 µL Reactions: 2000
• 20 µL Reactions: 5,000
• 10 µL Reactions: 10,000
• 5 µL Reactions: 20,000

4381657, TaqMan® Genotyping Master Mix, (2 x 50 mL)
• 50 µL Reactions: 4000
• 20 µL Reactions: 10,000
• 10 µL Reactions: 20,000
• 5 µL Reactions: 40,000

Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.


Technique: CNV (Copy Number Variation), SNP Genotyping (Real-Time PCR-Based)
PCR Method: qPCR
Polymerase: AmpliTaq Gold® DNA Polymerase
Product Size: 1 x 10 mL
Reaction Speed: Standard
Detection Method: Primer-Probe Detection
Genotyping Target: Copy Number Variants, SNPs (Known), Drug Metabolizing Enzymes, SNPs (Unknown or Numerous)
Passive Reference Dye: ROX (Pre-mixed)
Sample Type (General): DNA
Sample Type (Specific): DNA (Genomic)
Purity or Quality Grade: UP (Ultra Pure)
For Use With (Equipment): 7300 System, 7900HT System, 7000 System, 7500 System, StepOnePlus™, Standard Mode, StepOne™, Standard Mode
Primer-Probe Compatibility: TaqMan®
Shipping Condition: Wet Ice

Contents & storage

Supplied at 2X concentration. The mix is optimized for SNP genotyping using TaqMan® probes and contains AmpliTaq Gold® DNA Polymerase UP (Ultra Pure), dNTPs without dUTP, Passive Reference 1 and optimized mix components. This pack contains one 10 mL bottle, sufficient for 400 reactions at the 50 µL total volume.

Storage: 2-8°C

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).


Manuals & protocols

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