This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR™ Green I, VIC™/JOE™, and ROX™ dyes on the StepOne™ Real-Time PCR System.
Features of the StepOne™ Real-Time PCR System Spectral Calibration Kit include:
• 48-well optical reaction plates preloaded with dyes (FAM™/SYBR™ Green I, VIC™/JOE™, and ROX™) are provided for quick and easy calibration of the StepOne™ Real-Time PCR System • Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture • Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions
The Applied Biosystems™ StepOne™ Real-Time PCR System Spectral Calibration Kit contains one background plate and three preloaded, 48-well fast optical reaction plates containing five dye standards.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Calibrated Dye
FAM, JOE, ROX, VIC, SYBR™ Green
For Use With (Equipment)
StepOne™, Fast Mode, StepOne™, Standard Mode
Detection Method
Fluorescent
Product Line
StepOne™
Product Type
Spectral Calibration Kit
For Use With (Application)
Real Time PCR (qPCR)
Shipping Condition
Dry Ice
Calibration Type
Spectral Calibration
Contents & Storage
Contains three 48-well reaction plates—one background plate and two containing dye standards(plates I & II). Plate I contains FAM, JOE and ROX; Plate II contains SYBR™ Green I and VIC.
Store at -15 to 25°C. Protect from light.
Expiration date: 12 months from the date of delivery