Applied Biosystems™

StepOne™ Real-Time PCR System Spectral Calibration Kit (48 well)

Catalog number:  4371433

 Related applications:

Real Time PCR (qPCR)

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This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR® Green I, VIC®/JOE™, and ROX™ dyes on the StepOne™ Real-Time PCR System.

Features of the StepOne™ Real-Time PCR System Spectral Calibration Kit include:

• 48-well optical reaction plates preloaded with dyes (FAM™/SYBR® Green I, VIC®/JOE™, and ROX™) are provided for quick and easy calibration of the StepOne™ Real-Time PCR System
• Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions

The Applied Biosystems® StepOne™ Real-Time PCR System Spectral Calibration Kit contains one background plate and three preloaded, 48-well fast optical reaction plates containing five dye standards.
For Research Use Only. Not for use in diagnostic procedures.


Calibration Type: Spectral Calibration
Detection Method: Fluorescent
For Use With (Equipment): StepOne™, Fast Mode, StepOne™, Standard Mode
Label or Dye: FAM, JOE, ROX, SYBR® Green, VIC
Product Line: StepOne™
Product Size: 1 kit
Shipping Condition: Dry Ice

Contents & storage

Contains three 48-well reaction plates—one background plate and two containing dye standards(plates I & II). Plate I contains FAM, JOE and ROX; Plate II contains SYBR® Green I and VIC.

Store at -15 to 25°C. Protect from light.

Expiration date: 12 months from the date of delivery


Manuals & protocols