Applied Biosystems® TaqMan® RNase P Fast 48-Well Instrument Verification Plate is used to verify the performance of the Applied Biosystems® StepOne™ Real-Time PCR System. The TaqMan® RNase P Fast 48-Well Instrument Verification Plate is a 48-well PCR plate preloaded with the necessary reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each well contains preloaded reaction mix (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye–labeled probe) and template. The TaqMan® RNase P Fast 48-Well Instrument Verification Plate satisfies the same requirements as the Applied Biosystems® StepOne™ Real-Time PCR Systems installation. To pass installation, the instrument must demonstrate the ability to distinguish between 5,000 and 10,000 genomic equivalents with a 99.7% confidence level for a subsequent sample run in a single well. To ensure accurate results, Applied Biosystems® recommends that the performance of your instrument be verified every six months.