AmpFLSTR® MiniFiler™ PCR Amplification Kit

Catalog number: 4373872

Applied Biosystems™  Related applications: Forensics

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The AmpFLSTR® MiniFiler™ PCR Amplification Kit increases your ability to obtain DNA results from compromised samples that previously would have yielded limited or no genetic data. This means cold cases can come off the shelf for re-analysis and new, challenging samples have a better chance of delivering interpretable results. This is the world's first commercially available miniSTR kit.

Features of the AmpFLSTR® MiniFiler™ PCR Amplification Kit:

• A single amplification provides information on the eight largest and most difficult to amplify loci in the AmpFLSTR® Identifiler® and SGM Plus®.
• With amplicon sizes less than 270 bp, MiniFiler™ is optimized for use with the most difficult types of samples including those that are degraded and contain inhibitors.
• Ability to overcome the inhibitory effects of substances such as heme and humic acid which are commonly encountered in forensic samples.
• Can be used as an adjunct to other commercially available amplification kits to recover the larger loci, which cannot be amplified or that drop out.
• Utilizes proven 5-dye technology allowing compatibility of workflows with Identifiler®.

Five Dye Technology Allows Enhanced Throughput
MiniFiler™ utilizes the most advanced 5-dye technology to allow for the multiplex of more loci in a single lane or injection helping to increase your throughput and to simplify the workflow needed to obtain probative results. Our PET™ and LIZ™ dyes were specifically designed to expand the spectral range of the 4 dye system. We have expanded the spectral range to 660 nm allowing for maximum color separation with minimal spectral overlap. As with all of our products, the five-dye technology is validated for use with our latest instruments and software as a complete system.

Experience Matters™
When you partner with us you gain access to the most extensive knowledge and experience of our forensic support team and training resources dedicated to human identification laboratories. Our HID University™ provides continuing education for DNA professional, our support and Applications Scientist offer over 100 years combined forensic experience, and our extensive network of Field Service Engineers provide technical assistance with instrumentation.

Complete Validated Solution
We offer a complete solution for your human identification needs. Our system includes DNA quantification kits, a range of AmpFLSTR® STR kits, instrumentation, and consumables. Minifiler is part of the most comprehensively validated systems for STR-based Human Identification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations including: SWGDAM, DAB, CODIS, ENFSI, Interpol, and GITAD.

Note: For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

“This reagent is classified as a medical device in several countries. If it is not regulated as a medical device in specific region, it should be used for laboratory use only.”
For Forensic or Paternity Use Only.


Technique: STR (Short Tandem Repeat) Analysis
Polymerase: AmpliTaq Gold® DNA Polymerase
Label or Dye: NED™, VIC®, FAM™, PET™, LIZ™
Product Size: 100 reactions
Amplicon Size: <290 bp
Sample Volume: 25 µl⁄reaction
Reaction Format: SuperMix or Master Mix
Sample Type (Specific): DNA (Genomic), Forensic Sample, Forensic Sample (Highly Degraded)
For Use With (Equipment): GeneAmp™ 9700, 3100-Avant™ Genetic Analyzer, 310 Genetic Analyzer, GeneAmp® 9600, 3730 DNA Analyzer, 3130xl Genetic Analyzer, 3500 Genetic Analyzer, 3500xL Genetic Analyzer
Matrix and Size Standards: Matrix Standard Set DS-33, GeneScan™ 500 LIZ® Size Standard
Shipping Condition: Dry Ice

Contents & storage

• AmpFLSTR® MiniFiler™ Primer Set
• AmpFLSTR® MiniFiler™ Master Mix
• AmpFLSTR® MinFiler™ Allelic Ladder
Store at -20°C upon receipt, 2 to 8°C after initial use

• AmpFLSTR® Control DNA Male 007
Store at 2 to 8°C


Manuals & protocols