POROS™ MabCapture™ A Affinity Chromatography Resin
Product Image
Thermo Scientific™

POROS™ MabCapture™ A Affinity Chromatography Resin

Thermo Scientific™ POROS™ MabCapture™ A Media is a new design in Protein A chromatography resins technology, combining improvements made in bead and chemistry technology.
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantity
437473110 L
437473210 mL
437473050 mL
4374729250 mL
43747351 L
43747285 L
Catalog number 4374731
Price (USD)
-
Quantity:
10 L

POROS MabCapture A Media is a new design in Protein A chromatography resins technology, combining improvements made in bead and chemistry technology. These advances provide a monoclonal antibody purification resins that not only demonstrates the highest dynamic binding performance at low flow rates but also one that can maintain superior binding capacity at high linear velocities where other chromatography resins products cannot operate.

With POROS MabCapture affinity chromatography resins you can:

  • Obtain the highest dynamic binding capacity for monoclonal antibodies vs. conventional agarose or silica based Protein A medias
  • Maintain high dynamic binding capacity at flow rates over 700 cm/hr
  • Design processes with greater flexibility (shorter bed heights, faster flow rates or both) due to the improvements made to the antibody binding efficiency
  • Clean and sanitize with standard sodium hydroxide agents utilized for other chromatography products
  • Recognize major increases to process productivity and reduce the downstream bottleneck

The development of humanized monoclonal antibodies has continued to fuel the growth of the biotherapeutic market. Addressing the therapeutic demand for monoclonal antibodies has lead to major improvements to the cell based expression systems that are used to manufacture these proteins. Consequently, these improvements have moved the processing bottleneck from upstream cell expression to the downstream purification process, as the purification operations struggle to keep up with the large quantities of antibodies that need to be manufactured.

Protein A chromatography, which is used as the primary capture chromatography step in most antibody process platforms, is generally regarded as the major process bottleneck due to the slow flow rates and long residence times required for the effective operation of conventional Protein A chromatography medias.The introduction of POROS MabCapture chromatography resins is aimed at cracking open the downstream bottleneck and providing the capacity, efficiency and flexibility needed to make improvements to the downstream purification platform resulting in major increases to process productivity and antibody manufacturing.

Pharmaceutical Grade Reagent. For Manufacturing and Laboratory Use Only.
Specifications
Quantity10 L
Stationary PhaseProtein A
FormLiquid Suspension
Product LinePOROS™
TypeBulk Resin
Unit SizeEach
Contents & Storage
Store refrigerated at 2°C to 8°C.

Frequently asked questions (FAQs)

Are POROS analytical columns compatible with HPLC systems from Waters or Agilent?

All of the pre-packed POROS columns, regardless of column size or resin, have the same 10-32 female fittings on the column. Columns are compatible with Waters and Agilent HPLC systems. You will need 1/16 inch male fittings for the HPLC to attach the column.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

For any POROS analytical column, is the column compatible with an AKTA or Avant purification system?

All of the pre-packed columns, regardless column size or resin, have the same 10-32 female fittings on the column. They are all compatible with the AKTA instrument platform, however due to the column volume of the 2.1 mmD x 30 mmL columns (0.1 mL), these will not perform well in AKTA or Avant purification systems due to the relative size of the hold- up volume in these systems. The smallest column (PEEK or SS) that could feasibly be used in these systems is the 4.6mmD x 50mmL (0.8 mL) format.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Are the pre-packed POROS CaptureSelect or any POROS analytical columns compatible with an UPLC or is the use of these columns limited to HPLC?

UPLC is typically described as chromatography with sub-2 µm particles, which drives higher efficiency/better separation independent of operating flow rate. As particle size decreases, backpressure increases so the UPLC systems tend to have higher pressure ratings compared to HPLC systems. The flow rates on UPLC systems would be sufficient for POROS columns. Pump capabilities are typically 0.1-1.0 mL/min, so not as fast as a HPLC, but sufficient. Hold-up volumes on UPLC systems are lower than HPLCs, so this can help with sensitivity and efficiency. So from a pressure and flow standpoint there should be no challenge to operate a POROS CaptureSelect column on a UPLC system.

That being said, care should be taken running POROS columns on UPLC systems. Higher pressure safety interlock MUST be set at the rated column pressure, typically 180 bar. HPLC column hardware is not rated to operate at the static pressures possible and typical on UPLC systems. A clog or the like on a UPLC system could generate unsafe pressures on any HPLC-rated column before the default UPLC safety pressure interlock is tripped.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the physical or mechanical stability of POROS resins?

The physical stability of POROS resins is high. Customers have agitated, with overhead mixing and or bottom gassing for long periods of time (5-10 hours) and have performed hundreds of diaphragm pump passes during stall packs. Keep in mind that agitation with any device that creates grinding and sheer, such as with a magnetic stir bar, is not recommended for any type of resin.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What should I do if a POROS column dries out or air is pumped onto the column?

POROS resins rehydrate quite well. Here is the procedure that we recommend:

1. Flow 5% ethanol over the column at ?350 cm/hour for 3 CVs, followed by water or 0.1 M sodium chloride at 300 cm/hour for 3 CVs.
2. If baseline noise is still present, flow 20% ethanol over the column at 300 cm/hour for 3 CVs, followed by water or 0.1 M sodium chloride at 300 cm/hour for 3 CVs.
3. If you have established column qualification tests and specifications, perform the tests after rewetting.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all POROS™ MabCapture™ A Affinity Chromatography Resin FAQs