Applied Biosystems™

High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor

Catalog number:  4374966

 Related applications:

Reverse Transcription

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The High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor contains all components necessary for the quantitative conversion of up to 2 µg of total RNA to single-stranded cDNA in a single 20 µL reaction. This particular kit also includes 1 tubes of RNase inhibitor (200 µL).

The High Capacity cDNA Reverse Transcription Kit delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg total RNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives. Features of this kit include:

• Linear target amplification for real-time PCR
• Higher yields and precision than other cDNA synthesis kits—at a fraction of the cost
• 10-fold greater dynamic range than other kits

Extensively tested with a variety of templates
Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA.
For Research Use Only. Not for use in diagnostic procedures.


Reverse Transcriptase: MultiScribe™
Downstream Application: Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)
Reaction Format: Separate Components
Sample Type (General): RNA
Final Product: cDNA (First-Strand)
Final Product Size(s): 7 kb or less
Optimal Reaction Temperature: 37° C
Product Size: 200 reactions

Contents & storage

• 10X RT Buffer (1 mL)
• 10X RT Random Primers (1 mL)
• 25X dNTP Mix (100 mM, 0.2 mL)
• MultiScribe® Reverse Transcriptase (0.2 mL at 50 U/µL)
• RNase Inhibitor (1 × 0.2 mL at 20 U/µL)

Store between -15°C to -25°C.


Manuals & protocols