Applied Biosystems™

High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor

Catalog number: 4374967
Applied Biosystems™

High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor

Catalog number: 4374967
Catalog Number
4374967
Unit Size
1,000 reactions
Price (USD)
Price: 3,135.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
200 reactions
Price (USD)
Price: 676.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
43749671,000 reactions
Price: 3,135.00
Your Price:
***
4374966200 reactions
Price: 676.00
Your Price:
***
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The High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor contains all components necessary for the quantitative conversion of up to 2 µg of total RNA to single-stranded cDNA in a single 20 µL reaction. This particular kit also includes 5 tubes of RNase inhibitor (200 µL each).

The High Capacity cDNA Reverse Transcription Kit delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg total RNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.

Features of this kit include:

• Linear target amplification for real-time PCR
• Higher yields and precision than other cDNA synthesis kits at a fraction of the cost
• 10-fold greater dynamic range than other kits

Extensively tested with a variety of templates
Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)
Concentration
50 U/μL
Detection Method
Real Time PCR
Fidelity (vs. Taq)
25 X
Final Product Type
cDNA (First-Strand)
Format
Kit
GC-Rich PCR Performance
High
No. of Reactions
1000 Reactions
PCR Method
RT-PCR, RT-qPCR
Reaction Format
Separate components
Reaction Speed
Standard
Reagent Type
Reverse Transcription
Sample Type
RNA
Size (Final Product)
7 kb or Less
Starting Material
RNA
Technique
Reverse Transcription
Volume
100 μL, 20 μL
Includes
10X RT Buffer (2 x 1mL); 10X RT Random Primers (2 x 1mL); 25X dNTP Mix (100mM, 1mL); MultiScribe Reverse Transcriptase (1mL at 50U/μL); RNase Inhibitor (10 x 0.1mL at 20U/μL)
No. of Tests
1000 Reactions
Optimal Reaction Temperature
37°C
Product Type
High-Capacity cDNA Reverse Transcription Kit
Quantity
1000 Reactions
Reverse Transcriptase
MultiScribe™
Sufficient For
1000 Reactions

Contents & Storage

Contents:
• 10X RT Buffer (2 x 1 mL)
• 10X RT Random Primers (2 x 1 mL)
• 25X dNTP Mix (100 mM, 1 mL)
• MultiScribe™ Reverse Transcriptase (1 mL at 50 U/μL)
• RNase Inhibitor (5 x 0.2 mL at 20 U/μL)

Store between -15°C and -25°C.

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