Fast MicroSEQ® D2 rDNA Fungal Identification Kit, with protocol & quick reference card

Catalog number: 4382395

Applied Biosystems™  Related applications: Microbial Identification & Detection | Microbial Protein Production

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Description

This kit contains both the FAST MicroSEQ® D2 rDNA PCR and MicroSEQ® D2 rDNA Sequencing Kit for Fungal Identification. There are enough reagents for 50 identifications. The kit provides an easy-to-use DNA sequence-based method to identify most fungi. A protocol and Quick Reference Card are included.

• Sequence-based measurement ensures you get reproducible results in less than 24 hours.
• Growth requirement-independent measurement enables you to identify fungi using objective criteria.
• Universal domain-level primers eliminate up-front optimization.
• MicroSEQ® ID Analysis Software ensures accurate species identification.
• Customizable libraries give you the flexibility to create new libraries and search validated libraries that contain the most frequently encountered organisms.
• Requires minimal training; same work flow as the MicroSEQ® Full Gene 16S rDNA Bacterial Identification Kit.

Specific and Easy to Use
Based on phylogenetic analysis of the D2 region of large-subunit ribosomal RNA gene sequences, this easy-to-use kit enables you to identify even the most difficult fungi, yeast, and oomycete plant pathogens. To ensure accurate and specific results, the kit's software compares your samples to a database of D2 sequences from over 1,000 validated type species.

The MicroSEQ® ID System: A Complete, Integrated Solution
Streamlining every step in microbial identification, Applied Biosystems®MicroSEQ® System combines the advantages of MicroSEQ® ID Software with easy PrepMan Ultra sample preparation reagents and protocols, MicroSEQ® bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To ensure optimal performance and system-wide integration, we develop and test all components of the system together.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Target Organism Class: Fungi
Target Gene: D2 region of rRNA gene
Validated Application: Sequencing
Detection Method: Capillary Electrophoresis
For Use With (Equipment): GeneAmp® 9700, 3500 Genetic Analyzer, 3730xl DNA Analyzer, 3130 Genetic Analyzer, 3100-Avant™ Genetic Analyzer, 3100 Genetic Analyzer, 310 Genetic Analyzer, 3130xl Genetic Analyzer, 9800 Fast Thermal Cycler, 3730 DNA Analyzer, Veriti® Thermal Cycler, 3500xL Genetic Analyzer
Sample Type (Specific): DNA (Genomic)
Technique: SBT (Sequence-Based Typing)
Library Type: Customizable, Fungal Library (> 1100 species)
Product Size: 1 kit
Workflow Step: Cycle Sequencing, PCR Amplification
Target Molecule: DNA
Analysis Software: MicroSEQ® ID Analysis Software
Target Specificity: Target-Specific

Contents & storage

The kit contains enough reagent for 50 identifications:
PCR Master Mix (store at 2 to 6°C in PCR clean room)
Positive PCR Control DNA (store at 2 to 6°C in PCR clean room)
Negative PCR Control (store at 2 to 6°C in PCR clean room)
D2 Forward Sequencing Mix (store at -15 to -25°C)
D2 Reverse Sequencing Mix (store at -15 to -25°C)
Includes Protocol and Quick Reference Card

Documents

Manuals & protocols