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TaqMan® Array Human Stem Cell Pluripotency Panel

Catalog number: 4385344

Applied Biosystems™  Related applications: Cell Viability, Proliferation & Function | Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling | Stem Cell Analysis

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Description

The Applied Biosystems® TaqMan® Human Stem Cell Pluripotency Array is an efficient, easy-to-use micro fluidic card for the characterization of undifferentiated stem cells or their differentiated derivatives. This gene signature panel contains 90 well-defined genes validated as markers for pluripotency plus 6 endogenous controls. The format of the TaqMan Array allows for 1-4 replicates to be run in parallel per array. This product is sold in packages of 4 cards.

The Human Stem Cell Pluripotency Array contains a well-defined set of validated gene expression markers to characterize human embryonic stem (ES) cell identity and assess phenotypic variations between embryonic stem cell isolates. The gene content on this TaqMan Array resulted directly from the work of the I.S.C.I consortium and characterization of human embryonic stem cell lines by the International Stem Cell Initiative.

The array is pre-formatted and inventoried, cost-effective, convenient and easy to use without needing expensive robotics. Results are reproducible and consistent across samples, studies, and labs —the same data quality from card-to-card and lot-to-lot — even with different operators.

The Right Targets

These TaqMan Arrays use Applied Biosystem’s proprietary micro fluidics technology to simultaneously analyze gene expression levels by quantitative PCR for 92 test genes and 6 endogenous controls. Genes were included based on the following criteria:
    Expression in human embryonal carcinoma cells (hEC), primordial germ cells or human ES (hES) cells
    Known role in maintenance of pluripotency in hES cells
    Identification in microarray-based experiments as being correlated with the stem cell state
    Expression known to be characteristic of specific differentiation lineages in the post implantation embryo and mature tissues. These are the right targets to characterize embryonic stem cells.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Dried Down
Format: 384-well microfluidic card
Internal Probe Modification: FAM (5'), MGB (Minor Groove Binder) (3'), Non-Fluorescent Quencher (3')
Label or Dye: FAM™
Product Size: 4 cards
Reaction Speed: Standard
Research Category: Development, Reproduction, Stem Cell Research
Species: Human
Target Gene: 90 well-defined genes validated as markers for stem cell pluripotency

Contents & storage

Each TaqMan® Array well contains a single TaqMan® Assay for one 2 µL reaction. The cards should be stored at 4°C.

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Manuals & protocols

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