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      Applied Biosystems™

      TaqMan™ Array Human Stem Cell Pluripotency Panel

      Catalog number:  4385344

       Related applications:

      Cell Viability, Proliferation & Function

      | Real Time PCR (qPCR)

      | Real Time PCR-Based Gene Expression Profiling

      | Stem Cell Analysis

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      Description

      The Applied Biosystems® TaqMan® Human Stem Cell Pluripotency Array is an efficient, easy-to-use micro fluidic card for the characterization of undifferentiated stem cells or their differentiated derivatives. This gene signature panel contains 90 well-defined genes validated as markers for pluripotency plus 6 endogenous controls. The format of the TaqMan Array allows for 1-4 replicates to be run in parallel per array. This product is sold in packages of 4 cards.

      The Human Stem Cell Pluripotency Array contains a well-defined set of validated gene expression markers to characterize human embryonic stem (ES) cell identity and assess phenotypic variations between embryonic stem cell isolates. The gene content on this TaqMan Array resulted directly from the work of the I.S.C.I consortium and characterization of human embryonic stem cell lines by the International Stem Cell Initiative.

      The array is pre-formatted and inventoried, cost-effective, convenient and easy to use without needing expensive robotics. Results are reproducible and consistent across samples, studies, and labs —the same data quality from card-to-card and lot-to-lot — even with different operators.

      The Right Targets

      These TaqMan Arrays use Applied Biosystem’s proprietary micro fluidics technology to simultaneously analyze gene expression levels by quantitative PCR for 92 test genes and 6 endogenous controls. Genes were included based on the following criteria:
        Expression in human embryonal carcinoma cells (hEC), primordial germ cells or human ES (hES) cells
        Known role in maintenance of pluripotency in hES cells
        Identification in microarray-based experiments as being correlated with the stem cell state
        Expression known to be characteristic of specific differentiation lineages in the post implantation embryo and mature tissues. These are the right targets to characterize embryonic stem cells.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Form: Dried Down
      Format: 384-well microfluidic card
      Internal Probe Modification: FAM (5'), MGB (Minor Groove Binder) (3'), Non-Fluorescent Quencher (3')
      Label or Dye: FAM
      Product Line: TaqMan™
      Product Size: 4 cards
      Reaction Speed: Standard
      Research Category: Development, Reproduction, Stem Cell Research
      Species: Human
      Target Gene: 90 well-defined genes validated as markers for stem cell pluripotency

      Contents & storage

      Each TaqMan® Array well contains a single TaqMan® Assay for one 2 µL reaction. The cards should be stored at 4°C.

      Documents

      Manuals & protocols