Fast SYBR™ Green Master Mix
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Applied Biosystems™

Fast SYBR™ Green Master Mix

Fast SYBR™ Green Master Mix Real-Time PCR Master Mix Designed for Speed Obtain a fast, reliable, and cost-effective solution forRead more
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Catalog NumberQuantity
43856101 x 1 mL
43856121 x 5 mL
43856162 x 5 mL
43856175 x 5 mL
438561810 x 5 mL
43856141 x 50 mL
Catalog number 4385610
Price (USD)/ Each
144.00
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Quantity:
1 x 1 mL
Price (USD)/ Each
144.00
Add to cart
Fast SYBR™ Green Master Mix
Catalog number4385610
Price (USD)/ Each
144.00
-
Add to cart
Fast SYBR™ Green Master Mix Real-Time PCR Master Mix Designed for Speed Obtain a fast, reliable, and cost-effective solution for your real-time PCR applications without compromising sensitivity, specificity, dynamic range, or PCR efficiency.
• Fast—Real-time PCR results in as fast as 35 minutes
• Sensitive—Detect very low copies of target
• Specific—Minimize primer-dimer and non-specific amplification
• Reproducible—Consistent amplification across a wide dynamic range Fast SYBR™ Green Master Mix contains all of the components, excluding the template and primers, in a convenient 2X master mix. It includes the following components in an optimized buffer:
• AmpliTaq™ Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and enabling reactions to be set up at room temperature
• SYBR™ Green I dye to enable detection of double-stranded DNA
• Deoxynucleotides (dNTPs) to help maintain optimal PCR results
• Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination
• Passive internal reference based on proprietary ROX™ dye to enable increased precision

Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Fast SYBR Green Master Mix and added additional capabilities for your gene expression analysis.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodSYBR
FormatTube
GC-Rich PCR PerformanceHigh
PCR MethodqPCR
PolymeraseAmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP
Reaction SpeedFast
For Use With (Equipment)7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Label or DyeSYBR Green
Passive Reference DyeROX (Pre-mixed)
Product LineSYBR™
Product TypeReal Time PCR SYBR Master Mix
Purity or Quality GradeUP (Ultra Pure)
Quantity1 x 1 mL
Shipping ConditionDry Ice
Sufficient For100 Reactions of 20 μL
For Use With (Application)Gene Expression
Concentration2X
No. of Reactions100 Reactions
Sample TypeDNA (Genomic), cDNA
Volume1 mL
Unit SizeEach
Contents & Storage
1 x 1 mL vial. The 2X mix contains SYBR™ Green 1 Dye, AmpliTaq Gold™ Fast DNA Polymerase LD, dNTPs with dUTP⁄dTTP blend, Passive Reference 1 and optimized buffer components. 100 reactions in a 20 μL total volume. Store at -5 to -30°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).