Applied Biosystems™

Fast SYBR™ Green Master Mix

Catalog number: 4385618
Applied Biosystems™

Fast SYBR™ Green Master Mix

Catalog number: 4385618
Catalog Number
4385618
Unit Size
10 x 5 mL
Price (USD)
Availability
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Quantity
Catalog Number
Unit Size
1 x 1 mL
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Quantity
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Unit Size
1 x 5 mL
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Unit Size
2 x 5 mL
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5 x 5 mL
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Catalog Number
Unit Size
1 x 50 mL
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Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
438561810 x 5 mL
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43856101 x 1 mL
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43856121 x 5 mL
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43856162 x 5 mL
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43856175 x 5 mL
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43856141 x 50 mL
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Fast SYBR™ Green Master Mix Real-Time PCR Master Mix Designed for Speed Obtain a fast, reliable, and cost-effective solution for your real-time PCR applications without compromising sensitivity, specificity, dynamic range, or PCR efficiency.
• Fast—Real-time PCR results in as fast as 35 minutes
• Sensitive—Detect very low copies of target
• Specific—Minimize primer-dimer and non-specific amplification
• Reproducible—Consistent amplification across a wide dynamic range Fast SYBR™ Green Master Mix contains all of the components, excluding the template and primers, in a convenient 2X master mix. It includes the following components in an optimized buffer:
• AmpliTaq™ Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and enabling reactions to be set up at room temperature
• SYBR™ Green I dye to enable detection of double-stranded DNA
• Deoxynucleotides (dNTPs) to help maintain optimal PCR results
• Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination
• Passive internal reference based on proprietary ROX™ dye to enable increased precision

Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Fast SYBR Green Master Mix and added additional capabilities for your gene expression analysis.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
SYBR
Format
Vial
GC-Rich PCR Performance
High
PCR Method
qPCR
Polymerase
AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP
Reaction Speed
Fast
For Use With (Equipment)
7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Label or Dye
SYBR Green
Passive Reference Dye
ROX (Pre-mixed)
Product Line
SYBR™
Product Type
Real Time PCR SYBR Master Mix
Purity or Quality Grade
UP (Ultra Pure)
Quantity
10 x 5 mL
Sufficient For
5000 Reactions of 20 μL Each
For Use With (Application)
Gene Expression
Concentration
2X
No. of Reactions
5000 Reactions
Sample Type
DNA (Genomic), cDNA
Volume
10 x 5 mL

Contents & Storage

10 × 5 mL vials. The 2X mix contains SYBR™ Green 1 Dye, AmpliTaq Gold™ Fast DNA Polymerase LD, dNTPs with dUTP⁄dTTP blend, Passive Reference 1, and optimized buffer components. Sufficient for 5000 reactions of 20 μL each.

Store at -5 to -30°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

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