Applied Biosystems™

AgPath-ID™ One-Step RT-PCR Reagents

Catalog number: 4387391
Applied Biosystems™

AgPath-ID™ One-Step RT-PCR Reagents

Catalog number: 4387391
Catalog Number
4387391
Unit Size
1,000 reactions
Price (USD)
Price: 1,824.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
100 reactions
Price (USD)
Price: 229.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
500 reactions
Price (USD)
Price: 1,084.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
43873911,000 reactions
Price: 1,824.00
Your Price:
***
AM1005100 reactions
Price: 229.00
Your Price:
***
4387424500 reactions
Price: 1,084.00
Your Price:
***
Product Overview
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FAQ
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Additional Information
Recommendations
Recommendations
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
• Recommended for RNA pathogen amplification
• Consistent amplification of RNA targets with high specificity and sensitivity
• Optimized to work with your target-specific primers and probes
• Contains ROX for quantitative fluorescent signal normalization

AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time. Once assembled, results are available in approximately one hour. The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification. The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization.
WARNING: Cancer – www.P65Warnings.ca.gov

Specifications

Concentration
25X
Detection Method
Primer-Probe
Fidelity (vs. Taq)
25 X
Format
Tube
GC-Rich PCR Performance
High
No. of Reactions
1000 Reactions
PCR Method
1-step RT-qPCR
Polymerase
AmpliTaq Gold DNA Polymerase
Reaction Speed
Standard
Sample Type
RNA
Volume
14 mL
For Use With (Equipment)
7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
Passive Reference Dye
ROX (Pre-mixed)
Primer Probe Compatibility
TaqMan™
Product Line
AgPath-ID™, Ambion™
Product Type
One-Step RT-PCR Reagent
Remarks
Works on all AB platforms, but is optimized for the 7500.
Reverse Transcriptase
ArrayScript™
Shipping Condition
Dry Ice
Sufficient For
1,000 Reactions

Contents & Storage

Sufficient for 1000 reactions of 25 µL, contains:
• 14 mL 2X RT-PCR Buffer
• 1100 µL 25X RT-PCR Enzyme Mix
• 25 mL Nuclease-free Water.

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