Path-ID™ qPCR Master Mix with Manual

Catalog number: 4388643M

Applied Biosystems™  Related applications: Animal Health Real-Time PCR | Real Time PCR (qPCR)

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Description

Ambion® Path-ID™ qPCR Master Mix is a convenient enzyme, buffer, and dNTP mix for TaqMan® probe-based, real-time PCR detection of DNA targets. It is optimized for targets of importance in animal health and to function in the presence of PCR inhibitors. The mix is supplied in one tube suitable for 100 reactions. With the exception of PCR primers and probes for your target, the master mix contains everything you need for real-time PCR, including:
• AmpliTaq Gold® ultrapure thermostable DNA polymerase designed for automatic hot start, to minimize nonspecific PCR products
• Optimized buffer and dNTP concentration, for reliable PCR
• Passive internal reference, based on proprietary ROX™ dye, for precise data analysis.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Primer-Probe Detection
Reaction Speed: Standard, Fast
Form: Liquid
Format: Tube(s)
Hot Start: Built-In Hot Start
Polymerase: AmpliTaq Gold® DNA Polymerase
Product Size: 100 reactions
Number of Reactions: 100 Reactions
Passive Reference Dye: ROX (Pre-mixed)
Purity or Quality Grade: UP (Ultra Pure)
Primer-Probe Compatibility: TaqMan®

Contents & storage

2X qPCR Master Mix is stored at –20°C. Nuclease-free water may be stored at any temperature.

Documents

Manuals & protocols