Applied Biosystems™

High-Capacity RNA-to-cDNA™ Kit

Catalog number:  4388950

 Related applications:

PCR

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Description

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.

Features of this kit include:
Fast—short reaction time (typically 30–60 min)
Convenient—easy workflow with few pipetting steps
Reliable—reverse transcription of both abundant and limited targets
Flexible—optimized 2-step protocol, enabling multiple PCR reactions from a single reverse transcription reaction

Streamline your cDNA synthesis while maintaining detection sensitivity
The components of the two-tube kit work together to provide sensitive and specific reverse transcription across a broad range of template amounts. Amplification of a dilution series of the RNA concentration reference standard demonstrates exceptional reverse transcription and PCR efficiency across 11 orders of magnitude. This wide dynamic range allows one set of reaction conditions to detect transcripts from highly active as well as weakly expressed genes. The two-tube formulation reduces reaction time and requires fewer pipetting steps than with the High Capacity cDNA Reverse Transcription Kit, while maintaining high performance.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

PCR Method: 2-Step qRT-PCR
Reverse Transcriptase: MultiScribe™ MuLV
Hot Start: No
Sample Type (General): RNA
Product Line: Applied Biosystems™
Product Size: 500 reactions
Shipping Condition: Dry Ice

Contents & storage

• 1 x 500 µL enzyme mix (MuLV and RNase inhibitor protein) (20X)
• 5 x 1 mL RT Buffer Mix (2X) (includes dNTPs, random octamers, and oligo dT-16)

Documents

Manuals & protocols