Power SYBR® Green RNA-to-CT1-Step Kit

Catalog number: 4389986

Applied Biosystems™  Related applications: Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling

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Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction. The Power SYBR® Green RNA-to-CT1-Step kit has been formulated to ensure maximum sensitivity and reliability. The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets. It includes:
• AmpliTaq® Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation
• ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA
• An additive that reduces primer dimer formation, reducing non-specific products that can cause false positive signals
• RNase inhibitor, to prevent degradation of RNA templates
• A proprietary ROX™ dye that serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations for superb precision on Applied Biosystems® real-time PCR instruments.
For Research Use Only. Not for use in diagnostic procedures.


PCR Method: 1-Step qRT-PCR
Purity or Quality Grade: UP (Ultra Pure)
Reverse Transcriptase: ArrayScript™ UP
Polymerase: AmpliTaq Gold® DNA Polymerase
Detection Method: SYBR
Primer-Probe Compatibility: Unlabeled Oligos
Reaction Speed: Standard
For Use With (Equipment): StepOne™, Standard Mode, 7000 System, 7300 System, StepOnePlus™, Standard Mode, 7700 System, 7500 System, 7900HT System
Sample Type (General): RNA
Passive Reference Dye: ROX (Pre-mixed)
Label or Dye: SYBR® Green
Product Size: 200 reactions
Shipping Condition: Wet Ice

Contents & storage

Sufficient for 200 reactions at 50 µL reaction volume and includes:
• 1 x 5 mL tube of 2X master mix containing SYBR®Green 1 Dye, AmpliTaq Gold® DNA Polymerase UP, dNTPs, Passive Reference 1,and optimized buffer components. Store at 2-8°C after opening.
• 1 x 80 µL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15 to -30°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).


Manuals & protocols

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