Power SYBR™ Green RNA-to-CT™ 1-Step Kit
<i>Power</i> SYBR&trade; Green RNA-to-C<sub>T</sub>&trade; <i>1-Step</i> Kit
Applied Biosystems™

Power SYBR™ Green RNA-to-CT™ 1-Step Kit

사용이 쉬운 1단계 qRT-PCR 반응에서 민감도와 특이성 확보. Power SYBR™ Green RNA-to-CT™ 1-Step kit는 최대의 민감도와 신뢰성을 보장하도록 만들어졌습니다. 이자세히 알아보기
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카탈로그 번호반응 수
4389986500 Reactions
4391178100 Reactions
카탈로그 번호 4389986
제품 가격(KRW)
1,297,000
Each
카트에 추가하기
반응 수:
500 Reactions
제품 가격(KRW)
1,297,000
Each
카트에 추가하기
사용이 쉬운 1단계 qRT-PCR 반응에서 민감도와 특이성 확보. Power SYBR™ Green RNA-to-CT™ 1-Step kit는 최대의 민감도와 신뢰성을 보장하도록 만들어졌습니다. 이 새로운 제형은 primer dimer로 인한 거짓 양성 결과를 줄이고 데이터 정확성을 크게 개선시키며 적은 양의 표적도 믿을 수 있게 검출할 수 있습니다. 구성: • AmpliTaq™ Gold DNA Polymerase UP- hot start qPCR 및 비특이적 산물 형성 최소화 • ArrayScript™ UP Reverse Transcriptase, - 고수율 full-length cDNA를 생성하는 RT 조작 • 거짓 양성 결과를 야기하는 primer dimer 형성 및 비특이적 산물을 줄이는 첨가제 • RNase inhibitor- RNA templates 변성 방지 • 본사 고유의 ROX™ dye - Applied Biosystems™ real-time PCR 기기의 높은 정밀도를 위해 비-PCR-관련 형광 변동을 정규화하는 passive internal reference로 역할
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(장비)7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
반응 수500 Reactions
패시브 참조 염료ROX (Pre-mixed)
중합효소AmpliTaq Gold DNA Polymerase
제품라인RNA-to-CT, SYBR
제품 유형One-Step qRT-PCR Kit
순도 또는 품질 등급UP (Ultra Pure)
수량500 Reactions
역전사 효소ArrayScript™ UP
실행 시간Standard
샘플 종류RNA
배송 조건Wet Ice
충분500 Reactions at 20 μL
검출 방법SYBR
용도(애플리케이션)Gene Expression
라벨 또는 염료SYBR Green
PCR 방법1-step RT-qPCR
반응 속도Standard
Unit SizeEach
구성 및 보관
Sufficient for 500 reactions at 20 μL reaction volume and includes:
• 1 x 5 mL tube of 2X master mix containing SYBR™Green 1 Dye, AmpliTaq Gold™ DNA Polymerase UP, dNTPs, Passive Reference 1,and optimized buffer components. Store at 2-8°C after opening.
• 1 x 80 μL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15 to -30°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

자주 묻는 질문(FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.

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