Applied Biosystems™

Power SYBR™ Green RNA-to-CT1-Step Kit

Catalog number: 4389986
Applied Biosystems™

Power SYBR™ Green RNA-to-CT1-Step Kit

Catalog number: 4389986
Catalog Number
4389986
Unit Size
200 reactions
Price (USD)
Price: 752.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
40 reactions
Price (USD)
Price: 213.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
4389986200 reactions
Price: 752.00
Your Price:
-
439117840 reactions
Price: 213.00
Your Price:
-
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
Recommendations
Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction. The Power SYBR™ Green RNA-to-CT1-Step kit has been formulated to ensure maximum sensitivity and reliability. The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets. It includes:
• AmpliTaq™ Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation
• ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA
• An additive that reduces primer dimer formation, reducing non-specific products that can cause false positive signals
• RNase inhibitor, to prevent degradation of RNA templates
• A proprietary ROX™ dye that serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations for superb precision on Applied Biosystems™ real-time PCR instruments.
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
SYBR
No. of Reactions
200 Reactions
PCR Method
1-step RT-qPCR
Polymerase
AmpliTaq Gold DNA Polymerase
Purity
UP (Ultra Pure)
Reaction Speed
Standard
Sample Type
RNA
For Use With (Equipment)
7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Label or Dye
SYBR™ Green
Passive Reference Dye
ROX (Pre-mixed)
Primer Probe Compatibility
Unlabeled Oligos
Product Line
RNA-to-CT™, SYBR™
Product Type
One-Step qRT-PCR Kit
Purity or Quality Grade
UP (Ultra Pure)
Reverse Transcriptase
ArrayScript™ UP
Run Time
Standard
Shipping Condition
Wet Ice
Sufficient For
200 Reactions
For Use With (Application)
Gene Expression

Contents & Storage

Sufficient for 200 reactions at 50 µL reaction volume and includes:
• 1 x 5 mL tube of 2X master mix containing SYBR™Green 1 Dye, AmpliTaq Gold™ DNA Polymerase UP, dNTPs, Passive Reference 1,and optimized buffer components. Store at 2-8°C after opening.
• 1 x 80 µL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15 to -30°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).