dGTP BigDye® Terminator v3.0 Ready Reaction Cycle Sequencing Kit

Catalog number: 4390229

Applied Biosystems™  Related applications: DNA Sequencing | Sanger Sequencing

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The dGTP BigDye® Terminator Cycle Sequencing Kit's fluorescent dideoxy terminator chemistry is optimized for sequencing through GT- and G-rich templates.

• Ensure reliable results with difficult regions and finishing applications.
• Sequence templates with challenging sequence contexts.
• Sequence problematic templates that standard chemistries cannot.

Sequence Challenging Templates
The dGTP BigDye® Terminator Kit is ideal for working with GT- and G-rich and other difficult-to-sequence templates. The dGTP BigDye® Terminator Kit replaces dITP with dGTP and it enables you to extend through those difficult-to-sequence regions with confidence, avoiding early signal loss in these samples.

Optimized for GT-Rich Templates
Obtain higher success rate even with your difficult GT-rich templates. By using the same chemistries as the standard BigDye® Terminator Kits (only replacing dITP with dGTP) the dGTP BigDye® Terminator Kit provides proven performance, optimized for GT-rich and other difficult-to-sequence templates.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.


Recommended Applications: GT-Rich or Difficult Template Sequencing
Format: 12-strip wells, 384-well plate, 8-well strips, 96-well plate
Product Line: BigDye® Terminator, BigDye™
Product Size: 100 reactions
Technique: Fluorescent Dye Terminator Sequencing
Template Compatibility: BAC DNA, Genomic DNA (Bacterial), PCR Amplicons, Plasmid DNA (≤15Kb), Single Stranded DNA
Shipping Condition: Dry Ice

Contents & storage

The kit contains the AmpliTaq® DNA Polymerase, FS, BigDye
terminators v3.0, pGEM® - 3Zf(+) Control Template,
-21 M13 Control Primer (forward), and Premixed Ready Reaction.
Store kit at -15 to -25°C.


Manuals & protocols