Thermo Scientific™

SurePac™ Bio 550 SEC MDi™ HPLC Columns

Name: SurePac™ Bio 550 SEC MDi™ HPLC Columns
SKU: 43903-154631

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Catalog Number	Length (Metric)	Diameter (Metric)	Column Format	Flow Rate
43903-154631	150 mm	4.6 mm	Analytical Column	0.6 mL/min.
43903-152131	150 mm	2.1 mm	Analytical Column	125 μL/min.
43903-157831	150 mm	7.8 mm	Analytical Column	1.0 mL/min.
43903-032131	30 mm	2.1 mm	Guard Column	125 μL/min.
43903-034631	30 mm	4.6 mm	Guard Column	0.6 mL/min.
43903-037831	30 mm	7.8 mm	Guard Column	1.0 mL/min.

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Catalog number 43903-154631

Price (CLP)

Length (Metric):

150 mm

Diameter (Metric):

4.6 mm

Column Format:

Analytical Column

Flow Rate:

0.6 mL/min.

Experience the future of Size-Exclusion Chromatography (SEC) with SurePac™ Bio 550 SEC MDi™ analytical columns and guards exclusively designed to deliver robust and reproducible performance at high resolution and throughput to accelerate your therapeutics analysis process. The column's groundbreaking MDi™ technology integrates our advanced 3 μm monodisperse silica particle platform with bioinert hardware to ensure superior reliability for best sample recovery. SurePac™ Bio 550 SEC MDi™ column is ideal for AAV gene therapeutics analysis and offers excellent selectivity due to the dedicated smaller particle (3 μm) size combined with wide-pore (550Å) for separating monomeric AAVs from aggregates based on their size differences.

SurePac Bio 550 SEC MDi columns and guards are based on high-purity, spherical, porous (550 Å), 3 μm monodispersed silica particles that are covalently modified with a proprietary diol hydrophilic layer. This unique design yields shorter diffusion distances and enhanced reproducible mass transfer and narrower peaks. The consistent size of these particles facilitates precise control over media synthesis and column packing, significantly improving column-to-column and lot-to-lot reproducibility. The columns are housed in a hydrophilic bioinert-coated stainless-steel hardware to eliminate secondary interactions, ensuring optimal performance during the initial injection process.

Ideal for AAV gene therapy characterization and quality control assessment due to selectivity of the dedicated smaller particle (3 μm) combined with wide-pore (550Å) size
Superior column-to-column and lot-to-lot reproducibility eliminates column variability as a concern in stability and QA/QC testing
High-efficiency peaks with excellent recovery
Higher resolution and throughput for fast turnaround time without sacrificing performance
Bioinert hardware to eliminate analyte adsorption

Specifications

Column FormatAnalytical Column

Flow Rate0.6 mL/min.

Max. Pressure3500 psi (241 bar)

Packing MaterialMonodisperse Silica Resin

Quantity1 Ea.

Stationary PhaseProprietary

Temperature20°C to 60°C

Diameter (Metric)4.6 mm

Length (Metric)150 mm

Particle Size3 μm

PhaseSize Exclusion

TechniqueSEC

pH2 to 8

Unit SizeEach

Frequently asked questions (FAQs)

What types of column formats and flow conditions are available for SurePac Bio 550 SEC MDi columns, and how do I choose the right one?

The SurePac Bio 550 SEC MDi column is offered in multiple formats: e.g., 150 mm length × 4.6 mm ID (flow ~0.6 mL/min), 150 mm × 2.1 mm ID (flow ~125 µL/min), and guard formats (30 mm length) in various diameters.
Considerations for choosing the right format of SurePac Bio 550 SEC MDi column:
• Sample volume: larger diameter (4.6 and 7.8 mm) is compatible with larger sample volume, when you need to inject a lot of samples to achieve the required sensitivity
• System compatibility: Ensure your LC pump and detector make-up flow can support chosen flow rates without pressure issues.
• Solvent usage and dead volume: Smaller ID columns save solvent and reduce dilution—a factor in high-sensitivity labs.

What sample types or applications are well-suited for SurePac Bio 550 SEC MDi columns. Are there samples for which these columns are not the best choice?

SurePac Bio 550 SEC MDi columns are well-suited for:
• AAV (adeno-associated virus) monomer/aggregate separations in gene-therapy analytical labs.
• Biopharmaceuticals where high resolution of large biomolecules/aggregates is required.
• Large mRNA and DNA molecules
• Quality control or stability-testing workflows where reproducibility is critical.
The SurePac Bio 550 SEC MDi column may not be ideal if:
• SEC of small proteins because pores are too large
• You do not require the high resolution/throughput that 3 µm monodisperse columns provide. In such cases, a simpler, lower-cost SEC column might suffice.

What makes the MDi technology in SurePac Bio 550 SEC MDi columns different from conventional SEC column packings?

The MDi (“Monodisperse & inert hardware”) technology of the SurePac Bio 550 SEC MDi column incorporates monodisperse 3 µm silica particles (uniform size) plus bio-inert column hardware (to minimize undesired sample interactions).

Compared to standard polydisperse packings (e.g., 5 µm or larger silica particles, or less inert hardware), this leads to:
• Enhanced reproducibility (column-to-column, lot-to-lot)
• Sharper peaks (due to small particles)
• Faster separations at equal resolution compared to columns packed with traditional 5 µm particles (because smaller particles deliver higher plate counts with shorter columns)

What analytical challenges do SurePac Bio 550 SEC MDi columns help solve?

SurePac Bio 550 SEC MDi columns are size-exclusion HPLC columns designed for bio-therapeutic separations (notably AAV gene-therapy workflows) using a 3 µm monodisperse silica packing with ~550 Å pore size and bio-inert hardware. These columns help address challenges such as accurate separation of monomeric versus aggregated viral particles (or other large-biomolecule aggregates) with high resolution and reproducibility - a workflow where many conventional SEC columns may struggle due to broad peaks or secondary interactions.