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Applied Biosystems™

TaqMan™ PreAmp Master Mix (cDNA pre-amplification for qPCR)

Catalog number: 4391128
Applied Biosystems™

TaqMan™ PreAmp Master Mix (cDNA pre-amplification for qPCR)

Catalog number: 4391128
Catalog Number
4391128
Unit Size
40 reactions
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
200 reactions
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
439112840 reactions
-
4488593200 reactions
-
Product Overview
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TaqMan™ PreAmp Master Mix pre-amplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your precious sample into many more real-time PCR reactions.

TaqMan™ PreAmp Master Mix lets you:

• Analyze mRNA from precious samples such as laser capture microdissections, needle biopsies, and formalin-fixed paraffin-embedded (FFPE) tissues
• Stretch as little as 1 ng of cDNA into 200 real-time PCR reactions for gene expression analysis using TaqMan™ Gene Expression assays
• Analyze up to 100 gene expression targets with minimal hands-on time

Preserves Equilibrium of Targets Without Preamplification Bias
In the past, preamplification kits and techniques often resulted in uneven amplification of targets and incorrect or biased data (as shown by low correlation coefficients between preamplified and unamplified samples). TaqMan™ PreAmp Master Mix amplifies with no bias and provides extremely high correlation between amplified and unamplified cDNA.

Easy Workflow
With TaqMan™ PreAmp Master Mix, preamplification setup takes only a few minutes. After a one-time pooling of the TaqMan™ assays , preamplification setup (mixing the pooled assays with cDNA sample and TaqMan™ PreAmp Master Mix) takes only five additional minutes. Cycling takes 1.5 hours using a GeneAmp™ 9700 thermal cycler.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Primer-probe
GC-Rich PCR Performance
High
PCR Method
qPCR
Polymerase
AmpliTaq Gold DNA Polymerase
For Use With (Equipment)
7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Passive Reference Dye
No ROX
Product Line
TaqMan™
Product Type
PreAmp Master Mix
Sufficient For
40 Reactions
For Use With (Application)
Pre-amplification
Hot Start
Built-In Hot Start
No. of Reactions
40 Reactions
Sample Type
cDNA
Volume
1 mL

Contents & Storage

Contains 2X TaqMan™ PreAmp Master Mix (1 mL). Store at 2–8°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

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