Applied Biosystems™

TaqMan™ RNA-to-CT1-Step Kit

Catalog number:  4392653

 Related applications:

Real Time PCR (qPCR)

| Real Time PCR-Based Gene Expression Profiling

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Description

The Applied Biosystems TaqMan RNA-to-CT 1-Step Kit delivers consistent RNA target quantification for a wide variety of assays and is validated with Applied Biosystems comprehensive library of TaqMan Gene Expression assays. The kit is recommended for a variety of applications including general gene expression studies, biomarker discovery, and microarray validation.

Features of the TaqMan RNA-to-CT 1-Step Kit include:
• Accurate across a wide dynamic range for dependable performance
• Validated with TaqMan Gene Expression Assays for easy experimental setup
• Consistent performance with a wide variety of targets, including AT-rich, GC-rich and long sequences
• 1-Step format means fewer pipetting steps leading to less chances for errors

Efficiency across a broad range of input concentrations
Maximum flexibility in RNA template input quantity requires optimal real-time PCR efficiency across a broad range of template quantities. Figure 1 shows the excellent amplification efficiency across six orders of magnitude. The result is comparable to that of the TaqMan RNA-to-CT 2-Step Kit, and is a significant improvement in dynamic range and sensitivity over the TaqMan One-Step RT-PCR Master Mix Reagents.

High sensitivity at low target concentration
The sensitivity of TaqMan RNA-to-CT 1-Step Kit was validated using a synthetic RNA template for which copy number is precisely known. Significant sampling error occurs when measuring low quantities of target, so statistical analysis is required for proper evaluation using multiple replicates. Figure 2 shows the expected quantity of target and corresponding mean CT values. Statistical analysis indicates high confidence of sample quantification based on a T-test (Table I), consistent with as few as 10 copies of target*.

*Results are dependent on a variety of factors, including assay design.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

PCR Method: 1-Step qRT-PCR
Purity or Quality Grade: UP (Ultra Pure)
Reverse Transcriptase: ArrayScript™ UP
Polymerase: AmpliTaq Gold DNA Polymerase
Detection Method: Primer-Probe Detection
Primer-Probe Compatibility: TaqMan®
Reaction Speed: Standard
For Use With (Equipment): 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type (General): RNA
Passive Reference Dye: ROX (Pre-mixed)
Product Line: RNA-to-CT™, TaqMan™
Product Size: 40 reactions
Shipping Condition: Wet Ice

Contents & storage

Sufficient for 40 reactions at 50 µL reaction volume. Includes:
• 1 x 1 mL tube of 2X master mix containing AmpliTaq Gold® DNA Polymerase UP, dNTPs (including dUTP), Passive Reference 1, and optimized buffer components. Store at -15 to -25°C.
• 1 x 50 µL tube of 40X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15° to -30°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

Documents

Manuals & protocols