Fast MicroSEQ® D2 Fungal rDNA PCR Kit, with protocol & quick reference cards

Catalog number: 4396787

Applied Biosystems™  Related applications: Microbial Identification & Detection | Microbial Protein Production

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This kit is the PCR component of the FAST MicroSEQ® Fungal Identification System containing enough reagents for 50 PCR amplifications of fungal DNA unknowns. The sequencing component of the kit is required for species identification. Together, the two kits provide an easy-to-use DNA sequence-based method to identify most fungi.

• Sequence-based measurement ensures you get reproducible results in less than 24 hours.
• Growth requirement-independent measurement enables you to identify fungi using objective criteria.
• Universal domain-level primers eliminate up-front optimization.
• MicroSEQ ID Analysis Software ensures accurate species identification.
• Customizable libraries give you the flexibility to create new libraries and search validated libraries that contain the most frequently encountered organisms.
• Requires minimal training; same work flow as the MicroSEQ Full Gene 16S rDNA Bacterial Identification Kit.

Specific and Easy to Use
Based on phylogenetic analysis of the D2 region of large-subunit ribosomal RNA gene sequences, this easy-to-use kit enables you to identify even the most difficult fungi, yeast, and oomycete plant pathogens. To ensure accurate and specific results, the kit's software compares your samples to a database of D2 sequences from over 1,000 validated type species.

The MicroSEQ® ID System: A Complete, Integrated Solution
Streamlining every step in microbial identification, Applied Biosystems MicroSeq System combines the advantages of MicroSEQ ID Software with easy PrepMan Ultra sample preparation reagents and protocols, MicroSEQ bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To ensure optimal performance and system-wide integration, we develop and test all components of the system together.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.


Target Organism Class: Fungi
Target Gene: D2 region of rRNA gene
Validated Application: Polymerase Chain Reaction
Detection Method: Gel Electrophoresis
For Use With (Equipment): GeneAmp™ 9700, Veriti® Thermal Cycler, 9800 Fast Thermal Cycler
Sample Type (Specific): DNA (Genomic)
Technique: SBT (Sequence-Based Typing)
Library Type: Customizable, Fungal Library (> 1100 species)
Product Size: 1 kit
Workflow Step: PCR Amplification
Analysis Software: MicroSEQ® ID Analysis Software

Contents & storage

The kit contains enough reagent for 50 identifications:
Store at -15 to -25°C upon reciept. After first use, store at 2-6 °C in a PCR Clean Room.
Includes Protocol and Quick Reference Card


Manuals & protocols