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Applied Biosystems™
AmpliTaq™ 360 DNA Polymerase
Description
The Applied Biosystems AmpliTaq360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. Compared to the original AmpliTaq DNA polymerase, AmpliTaq 360 polymerase is purified by an additional proprietary separation process which reduces contaminating bacterial DNA sequences from the enzyme preparation. This ultra-pure enzyme reduces false positive results, amplifies low-level target sequences, and when combined with the proprietary AmpliTaq 360 Buffer Kit, promotes the amplification of a variety of templates including those from bacterial and human genomes.• Optimized for the broadest range of targets—from everyday to challenging
• Unmatched sensitivity and yield
• Market-leading GC enhancer for robust amplification of GC-rich sequences
• Achieves the highest quality sequencing data
For superior PCR performance, we recommend DreamTaq DNA Polymerase.
For Research Use Only. Not for use in diagnostic procedures.
Figures
Table 1. Average Specific Yield and Specificity for AmpliTaq® 360 DNA Polymerase Compared with Promega GoTaq® Colorless DNA Polymerase.
Figure 2a. AmpliTaq® 360 DNA Polymerase Demonstrates Overall Superior Specificity and Yield.
Figure 1. AmpliTaq® 360 DNA Polymerase Amplifies a Broad Range of Targets.
Figure 2b. AmpliTaq® 360 DNA Polymerase Demonstrates Overall Superior Specificity and Yield.
Figure 4. Sensitivity of AmpliTaq® 360 DNA Polymerase for Detection of Low Copy Targets.
Figure 5. High Quality Overall Sequencing Data Obtained from AmpliTaq® 360 DNA Polymerase-Generated PCR Products.
Figure 3. Amplification of Long Targets with AmpliTaq® 360 DNA Polymerase.
Specifications
| Exonuclease Activity: | 5' - 3' |
|---|---|
| Fidelity (vs. Taq): | 1 X |
| Final Product Size(s): | 5 kb or less |
| GC-Rich PCR Performance: | Medium |
| Green Features: | Sustainable packaging |
| Hot Start: | No |
| Polymerase: | AmpliTaq® 360 DNA Polymerase |
| Product Line: | AmpliTaq™ |
| Product Overhang: | 3'-A |
| Product Size: | 1,000 units |
| Reaction Format: | Separate Components |
| Reaction Speed: | Standard |
| Shipping Condition: | Approved for shipment at Room Temperature or on Wet Ice |
Contents & storage
AmpliTaq® 360 DNA Polymerase is supplied at 5U⁄µL with 10X AmpliTaq® 360 PCR Buffer, 25mM M2Cl2 , and 360 GC Enhancer. Typically, 1.5mL of each of AmpliTaq 360 Buffer, 25mM MgCl2 , and 360 GC Enhancer is supplied with each 250U of enzyme.
• Storage Buffer Enzyme is supplied in 20 mM Tris, pH 9.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% (w⁄v) Tween 20,
50% (v⁄v) glycerol
• Source: Recombinant, modified form of the Thermus aquaticus DNA polymerase gene expressed in E. coli
All solutions are prepared in deionized, filter-sterilized water. When stored at -15 to -25 °C, this product maintains performance at least until the expiration date on the tube label. Before use, thaw frozen components to room temperature and mix well.
Product is guaranteed up to 12 months as indicated by the expiration date.
• Storage Buffer Enzyme is supplied in 20 mM Tris, pH 9.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% (w⁄v) Tween 20,
50% (v⁄v) glycerol
• Source: Recombinant, modified form of the Thermus aquaticus DNA polymerase gene expressed in E. coli
All solutions are prepared in deionized, filter-sterilized water. When stored at -15 to -25 °C, this product maintains performance at least until the expiration date on the tube label. Before use, thaw frozen components to room temperature and mix well.
Product is guaranteed up to 12 months as indicated by the expiration date.
