AmpliTaq® 360 Buffer, 25 mM MgCl2 and 360 GC Enhancer This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: 4398848

Applied Biosystems™  Related applications: PCR

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Description

The Applied Biosystems® AmpliTaq® 360 Buffer Kit is used in conjection with the AmpliTaq 360 DNA polymerase for those few experiments when the reagents supplied with the enzyme are not sufficient for the researcher's needs. When used together with or without the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. AmpliTaq® 360 DNA Polymerase delivers 360° coverage for a full range of targets.

* Optimized for the broadest range of targets—from everyday to challenging
* Robust amplification of GC-rich sequences with market-leading 360 GC Enhancer
* Achieves the highest quality sequencing data

Optimized for Easy and Challenging Targets
Challenging targets include AT-rich, GC-rich, primer-dimer forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions (Figure 1). Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold® 360 as the best-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets (Table 1).

As shown in Figure 1, GC-rich regions are especially poorly amplified with competitor DNA polymerases and the original AmpliTaq Gold, while AmpliTaq Gold 360 provides successful, robust amplification.

Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: AmpliTaq® 360 DNA Polymerase
Product Size: 1.5 mL
Green Features: Less waste, Sustainable packaging
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice

Contents & storage

AmpliTaq® 360 Buffer kit is supplied 1.5 mL of each 10X AmpliTaq® 360 Buffer, 25mM M2Cl2 , and 360 GC Enhancer. Typically, 1.5mL of each of AmpliTaq 360 Buffer, 25mM MgCl2 , and 360 GC Enhancer is sufficient for use with 250U of enzyme.

All solutions are prepared in deionized, filter-sterilized water. When stored at -15 to -25 °C, this product maintains performance at least until the expiration date on the tube label. Before use, thaw frozen components to room temperature and mix well.

Product is guaranteed up to 12 months as indicated by the expiration date.

Documents

Manuals & protocols