AmpliTaq Gold™ 360 Buffer Kit

El kit de tampón AmpliTaq Gold™ 360 se utiliza junto con la ADN polimerasa AmpliTaq Gold™ 360 y está diseñadoMás información

Número de catálogo	Cantidad
4398853	1 kit

Número de catálogo 4398853

Precio (MXN)

Cantidad:

1 kit

Pedido a granel o personalizado

El kit de tampón AmpliTaq Gold™ 360 se utiliza junto con la ADN polimerasa AmpliTaq Gold™ 360 y está diseñado para aquellas ocasiones en las que la cantidad de reactivos suministrados con la enzima no sea suficiente. El kit incluye 1,5 ml (cada uno) de tampón AmpliTaq Gold 360 10X, 25 mm de MgCl₂ y potenciador 3360 GC. Por lo general, 1,5 ml es suficiente para utilizar con 250 U de enzima.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Fidelidad (frente a Taq)10 X

FormatoKit

PolimerasaADN polimerasa AmpliTaq Gold 360

Tipo de productoTampón PCR

Cantidad1 kit

Condiciones de envíoAprobado para su envío a temperatura ambiente o en hielo húmedo

Volumen1,5 mL

Concentración10X

Para utilizar con (aplicación)Clonación

Unit SizeEach

Contenido y almacenamiento

• 1 x 1,5 ml tampón AmpliTaq Gold 360, 10X
• 1 x 1,5 ml, 25 mm de MgCl₂
• 1 x 1,5 ml de potenciador 360 GC

Almacenar a una temperatura entre -15 °C y -25 °C.

Preguntas frecuentes

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

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