AmpliTaq® 360 DNA Polymerase This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: 4398893

Applied Biosystems™  Related applications: PCR

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Description

The Applied Biosystems® AmpliTaq® 360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. Compared to the original AmpliTaq® DNA polymerase, AmpliTaq® 360 polymerase is purified by an additional proprietary separation process which reduces contaminating bacterial DNA sequences from the enzyme preparation. This ultra-pure enzyme reduces false positive results, amplifies low-level target sequences, and when combined with the proprietary AmpliTaq® 360 Buffer Kit, promotes the amplification of a variety of templates including those from bacterial and human genomes.

* Optimized for the broadest range of targets—from everyday to challenging
* Unmatched sensitivity and yield
* Market-leading GC enhancer for robust amplification of GC-rich sequences
* Achieves the highest quality sequencing data

Extensively Tested
AmpliTaq® 360 DNA Polymerase has been extensively tested and optimized across a broad panel of difficult targets for best-in-class performance. Challenging targets include AT-rich, GC-rich, primer-dimer forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can now be amplified reproducibly with a single reagent under standardized conditions.

Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq 360 as the best-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets (Figure 1). As shown in Figure 1, GC-rich regions are poorly amplified with other DNA polymerases while AmpliTaq 360 DNA Polymerase provides successful, robust amplification. These data are shown in a graphical format for specificity (Figure 2a) and specific yield (Figure 2b). The data averaged over all the targets is summarized in Table 1.

Superior Length and Sensitivity
AmpliTaq 360 DNA Polymerase reproducibly and efficiently amplifies long (up to 5 Kb) sequences. Figure 3 demonstrates high quality PCR amplification of long human and plasmid DNA. AmpliTaq 360 DNA Polymerase efficiently amplifies targets present at low copy number (Figure 4), even in the presence of high concentrations of complex DNA, making it especially suited for low-copy pathogen detection, multiplex PCR, allelic discrimination and amplification of targets from degraded DNA samples. The extreme purity of the enzyme, along with its validated and optimized buffer system, contributes to its unmatched sensitivity.

Superior Sensitivity, Reproducibility and Yield
Compared to the original AmpliTaq® DNA Polymerase, AmpliTaq® 360 DNA Polymerase is purified by an additional proprietary separation process which reduces contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Buffer, this ultra-pure enzyme, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates including those from bacterial and human genomes. This provides excellent specificity, reproducibility and yield across a broad range of targets (Figure 1).

Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: AmpliTaq® 360 DNA Polymerase
Hot Start: No
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Medium
Reaction Format: Separate Components
Product Overhang: 3'-A
Reaction Speed: Standard
Final Product Size(s): 5 kb or less
Exonuclease Activity: 5' - 3'
Product Line: AmpliTaq®, Applied Biosystems™
Product Size: 3,000 units
Green Features: Less waste, Sustainable packaging

Contents & storage

AmpliTaq® 360 DNA Polymerase is supplied at 5U⁄µL with 10X AmpliTaq® 360 PCR Buffer, 25mM M2Cl2 , and 360 GC Enhancer. Typically, 1.5mL of each of AmpliTaq 360 Buffer, 25mM MgCl2 , and 360 GC Enhancer is supplied with each 250U of enzyme.
• Storage Buffer Enzyme is supplied in 20 mM Tris, pH 9.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% (w⁄v) Tween 20,
50% (v⁄v) glycerol
• Source: Recombinant, modified form of the Thermus aquaticus DNA polymerase gene expressed in E. coli

All solutions are prepared in deionized, filter-sterilized water. When stored at -15 to -25 °C, this product maintains performance at least until the expiration date on the tube label. Before use, thaw frozen components to room temperature and mix well.

Product is guaranteed up to 12 months as indicated by the expiration date.

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