Invitrogen™

Power SYBR™ Green Cells-to-CT™ Kit

Green features
Catalog number: 4402954
Invitrogen™

Power SYBR™ Green Cells-to-CT™ Kit

Green features
Catalog number: 4402954
Catalog Number
4402954
Unit Size
100 reactions
Price (USD)
1,332.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
400 reactions
Price (USD)
4,250.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
40 reactions
Price (USD)
616.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
4402954100 reactions
1,332.00
Your Price:
***
4402955400 reactions
4,250.00
Your Price:
***
440295340 reactions
616.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Ambion® Power SYBR® Green Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results. A breakthrough cell lysis and RNA stabilization technology eliminates the laborious and time-consuming RNA purification process completely. The kit includes sufficient reagents for 400 reactions. Power SYBR® Green Cells-to-CT™ Kit is Powered by AmpliTaq Gold® DNA Polymerase, LD, which provides the highest levels of specificity with standard real-time PCR.

• Includes optimized reagents to work efficiently, right out of the box
• Simple, effective Cells-to-CT™ technology enables sample preparation at room temperature in only 7 minutes, including DNase treatment
• Validated accuracy, exceptional reproducibility, and maximal sensitivity from 10 to 100,000 cells per sample; results equivalent to those from purified RNA

Extraordinary Value
The kit includes reagents for sample preparation integrated into a complete, optimized gene expression workflow including reverse transcription reagents and a high-performance PCR master mix with SYBR® Green.

Extraordinary Ease
The protocol begins with a simple 7-minute sample preparation procedure. First, cells are washed in PBS, and then lysed for 5 minutes at room temperature; DNase treatment can be performed concurrently. The lysis reaction is stopped, and the sample is then ready for reverse transcription or storage at –20°C for up to 5 months. Because samples are processed directly in culture plates (96- or 384-well), sample handling and the potential for sample loss or transfer error are minimized, resulting in higher reproducibility. Following sample preparation, a portion of the cell lysate is added to an RT reaction followed by real-time PCR using the included master mix.

Extraordinary Performance
To demonstrate the performance of the kit, replicate experiments were run using either traditional RNA purification and real-time PCR analysis, or the Power SYBR® Green Cells-to-CT™ Kit. The kit shows equivalent performance to results obtained using purified RNA for analysis of 10 to 100,000 cells. In addition, the sensitivity, efficiency, and dynamic range were shown to be superior to competitor lysate kits. Samples prepared with the kit have been tested for compatibility on various real-time PCR platforms including those from Applied Biosystems®, Roche, and Bio-Rad.

Accessory Product:
The SYBR® Green Cells-to-CT™ Control Kit (SKU# 4402959) contains a Xeno™ RNA control and primer set and is recommended to monitor the efficiency of cell lysis and amplification inhibition in Cells-to-CT™ reactions.
WARNING: Cancer and Reproductive Harm – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
20X
Quantity
100 reactions
Hot Start
Built-In Hot Start
Passive Reference Dye
ROX (Pre-mixed)
PCR Method
2-Step qRT-PCR
Polymerase
AmpliTaq Gold DNA Polymerase
Primer Probe Compatibility
Unlabeled Oligos
Purity or Quality Grade
LD (Low DNA)
Reaction Speed
Standard
Reverse Transcriptase
M-MLV
Shipping Condition
Dry Ice
Product Line
Cells-to-CT™, SYBR™
For Use With (Equipment)
7000 System, 7200 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 5700, GeneAmp 9600, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Label or Dye
SYBR™ Green
Sample Type
Cells
Green Features
Fewer resources used and less waste, Less hazardous
Detection Method
SYBR
Purification Time
5 min
No. of Reactions
100 Reaction

Contents & Storage

Store Stop Solution, DNase I and 20X RT Enzyme Mix at -20°C. Store Lysis Solution, 2X SYBR™RT Buffer, and Power SYBR™Green PCR Master Mix at 4°C. Store Nuclease–free Water at room temperature.

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