Applied Biosystems™

TaqMan™ Sample-to-SNP™ Kit (20 mL sample prep, 10 mL PCR)

Catalog number:  4403083

 Related applications:

Genotyping & Genomic Profiling

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The Applied Biosystems® TaqMan® Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than one hour. The TaqMan® Sample-to-SNP™ Kit combines DNA extraction and TaqMan® chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

• DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
• The TaqMan®™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2°–8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays
• Extended PCR cycling for poorly amplified or sample-limited reactions

The kit includes reagents sufficient for 400 sample preparation reactions at 50 µL/ reaction and 4,000 PCR reactions at 5 µL for each reaction.

Please note:
The DNA Extract All reagents are not available for individual sale. They are only available as a part of the TaqMan® Sample-to-SNP™ Kit.
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Primer-Probe Detection
For Use With (Equipment): 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, GeneAmp 9700, StepOne™, Standard Mode, Veriti Thermal Cycler
Genotyping Target: SNPs (Known), SNPs (Unknown or Numerous)
Hot Start: Built-In Hot Start
PCR Method: qPCR
Passive Reference Dye: ROX (Pre-mixed)
Polymerase: AmpliTaq® DNA Polymerase
Primer-Probe Compatibility: TaqMan®
Product Line: Sample-to-SNP™, TaqMan™
Product Size: 1 kit
Reaction Speed: Fast, Standard
Sample Type (General): Blood, Buccal Samples, Cells, Plant Samples, Tissue
Sample Type (Specific): Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood
Technique: SNP Genotyping (Real-Time PCR-Based)

Contents & storage

Contains Lysis Reagents (2 -8°C): 20 mL each Lysis and DNA Stabilization Solution; Master Mix (15 to 25°C): 10 mL 2X master mix with AmpliTaq® Fast DNA Polymerase, UP, dNTPs, Tracking dye and ROX™ Dye.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).


Manuals & protocols