TaqMan™ Sample-to-SNP™ Kit (20 mL sample prep, 50 mL PCR)

The Applied Biosystems TaqMan Sample-to-SNP Kit allows you to go from biological sample to genotyping results in less than one詳細を見る

製品番号（カタログ番号）	数量
4403087	1 kit

製品番号（カタログ番号） 4403087

価格（JPY）

833,700

お問い合わせください ›

1 kit

The Applied Biosystems TaqMan Sample-to-SNP Kit allows you to go from biological sample to genotyping results in less than one hour. It combines DNA extraction and TaqMan chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

• Provides PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
• The TaqMan GTXpress Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2–8°C significantly reduce assay setup time
• Validated with all types of TaqMan Genotyping assays
• Extended PCR cycling for poorly amplified or sample-limited reactions

The kit includes reagents sufficient for 400 sample preparation reactions at 50 μL/reaction and 20,000 PCR reactions at 5 μL for each reaction.

研究用途にのみご使用ください。診断目的には使用できません。

使用対象 (装置)7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, GeneAmp 9700, StepOne™, Fast Mode, StepOne™, Standard Mode, Veriti Thermal Cycler, StepOne™ Standard Mode, StepOne™ Fast Mode

ジェノタイピングターゲットSNPs (Unknown or Numerous), SNPs (Known)

ホットスタート内蔵ホットスタート

反応数4000 Reactions (5 μL, PCR), 4000 Reactions (50 μL/Reaction, Sample Preparation)

受動的参照色素ROX（プレミックス）

ポリメラーゼAmpliTaq DNA Polymerase

製品ラインSample-to-SNP, TaqMan

製品タイプPCR Kit

数量1 kit

サンプルタイプBuccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood

技術SNP Genotyping (Real-Time PCR-Based)

容量20 mL (Sample Preparation), 50 mL (PCR)

検出法Primer-probe

PCR法qPCR

反応速度高速または標準

Unit SizeEach

組成および保存条件

Contains Lysis Reagents (2 -8°C): 20 mL each Lysis and DNA Stabilization Solution; Master Mix (15 to 25°C): 50 mL 2X master mix with AmpliTaq™ Fast DNA Polymerase, UP, dNTPs, Tracking dye and ROX™ Dye.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

よくあるご質問（FAQ）

When designing probes to perform Allelic Discrimination (SNP assays), are there any alternatives to using probes longer than 30bp in order to get the appropriate melting temperature?

Applied Biosystems TaqMan MGB (minor groove binder) probes would be appropriate for Allelic Discrimination/SNP Genotyping probe designs. TaqMan MGB probes have a minor groove binder at the end of the probe. This minor groove binder increases the Tm of probes, allowing the use of shorter probes. Consequently, the TaqMan MGB probes exhibit greater differences in Tm values between matched and mismatched probes, which provides more accurate allele discrimination.

For information on the design of TaqMan MGB probes for Allelic Discrimination/SNP assays using Primer Express software, please refer to our guide "Designing TaqMan MGB Probe and Primer Sets for Allelic Discrimination Assays Using Primer Express".