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Applied Biosystems™

TaqMan™ Sample-to-SNP™ Kit (20 mL sample prep, 50 mL PCR)

The Applied Biosystems TaqMan Sample-to-SNP Kit allows you to go from biological sample to genotyping results in less than one자세히 알아보기
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카탈로그 번호수량
44030871 kit
카탈로그 번호 4403087
제품 가격(KRW)
7,380,000
온라인 행사
Ends: 30-Jun-2026
8,200,000
할인액 820,000 (10%)
Each
수량:
1 kit
제품 가격(KRW)
7,380,000
온라인 행사
Ends: 30-Jun-2026
8,200,000
할인액 820,000 (10%)
Each

The Applied Biosystems TaqMan Sample-to-SNP Kit allows you to go from biological sample to genotyping results in less than one hour. It combines DNA extraction and TaqMan chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

• Provides PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
• The TaqMan GTXpress Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2–8°C significantly reduce assay setup time
• Validated with all types of TaqMan Genotyping assays
• Extended PCR cycling for poorly amplified or sample-limited reactions

The kit includes reagents sufficient for 400 sample preparation reactions at 50 μL/reaction and 20,000 PCR reactions at 5 μL for each reaction.

For Research Use Only. Not for use in diagnostic procedures.
사양
용도(장비)7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, GeneAmp 9700, StepOne™, Fast Mode, StepOne™, Standard Mode, Veriti Thermal Cycler, StepOne™ Standard Mode, StepOne™ Fast Mode
유전형 분석 타겟SNPs (Unknown or Numerous), SNPs (Known)
핫 스타트Built-In Hot Start
반응 수4000 Reactions (5 μL, PCR), 4000 Reactions (50 μL/Reaction, Sample Preparation)
패시브 참조 염료ROX (Pre-mixed)
중합효소AmpliTaq DNA Polymerase
제품라인Sample-to-SNP, TaqMan
제품 유형PCR Kit
수량1 kit
샘플 종류Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood
기술SNP Genotyping (Real-Time PCR-Based)
부피20 mL (Sample Preparation), 50 mL (PCR)
검출 방법Primer-probe
PCR 방법qPCR
반응 속도Fast or Standard
Unit SizeEach
구성 및 보관
Contains Lysis Reagents (2 -8°C): 20 mL each Lysis and DNA Stabilization Solution; Master Mix (15 to 25°C): 50 mL 2X master mix with AmpliTaq™ Fast DNA Polymerase, UP, dNTPs, Tracking dye and ROX™ Dye.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

자주 묻는 질문(FAQ)

When designing probes to perform Allelic Discrimination (SNP assays), are there any alternatives to using probes longer than 30bp in order to get the appropriate melting temperature?

Applied Biosystems TaqMan MGB (minor groove binder) probes would be appropriate for Allelic Discrimination/SNP Genotyping probe designs. TaqMan MGB probes have a minor groove binder at the end of the probe. This minor groove binder increases the Tm of probes, allowing the use of shorter probes. Consequently, the TaqMan MGB probes exhibit greater differences in Tm values between matched and mismatched probes, which provides more accurate allele discrimination.

For information on the design of TaqMan MGB probes for Allelic Discrimination/SNP assays using Primer Express software, please refer to our guide "Designing TaqMan MGB Probe and Primer Sets for Allelic Discrimination Assays Using Primer Express".