Applied Biosystems™

TaqMan™ Sample-to-SNP™ Kit (20 mL sample prep, 50 mL PCR)

The Applied Biosystems TaqMan Sample-to-SNP Kit allows you to go from biological sample to genotyping results in less than one hour.

Número de catálogo	Cantidad
4403087	1 kit

Número de catálogo 4403087

Precio (MXN)

Cantidad:

1 kit

The Applied Biosystems TaqMan Sample-to-SNP Kit allows you to go from biological sample to genotyping results in less than one hour. It combines DNA extraction and TaqMan chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

Provides PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
The TaqMan GTXpress Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
Compatible with both Fast and Standard mode thermal cycling conditions
Pre-mixed components stored at 2–8°C significantly reduce assay setup time
Validated with all types of TaqMan Genotyping assays
Extended PCR cycling for poorly amplified or sample-limited reactions

The kit includes reagents sufficient for 400 sample preparation reactions at 50 μL/reaction and 20,000 PCR reactions at 5 μL for each reaction.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Para utilizar con (equipo)Sistema 7300, sistema 7500 Fast, sistema 7500, sistema 7900HT Fast, GeneAmp 9700, StepOne™, modo rápido, StepOne™, modo estándar, termociclador Veriti, StepOne™ modo estándar,StepOne™ modo rápido

Objetivo de genotipadoSNP (desconocidos o numerosos), SNP (conocidos)

Inicio en calienteBuilt-In Hot Start

N.º de reacciones4000 Reactions (5 μL, PCR), 4000 Reactions (50 μL/Reaction, Sample Preparation)

Colorante de referencia pasivaROX (premezclado)

PolimerasaAmpliTaq DNA Polymerase

Línea de productosSample-to-SNP, TaqMan

Tipo de productoPCR Kit

Cantidad1 kit

Tipo de muestraMuestra bucal, cultivos celulares, cabello con folículo, perforación de hoja o aguja, viruta de semilla, biopsia de cola (ratón), biopsia de cola (rata), tejido (FFPE), sangre completa

TécnicaSNP Genotyping (Real-Time PCR-Based)

Volumen20 mL (Sample Preparation), 50 mL (PCR)

Método de detecciónSonda de cebado

Método de PCRqPCR

Velocidad de reacciónrápida o estándar

Unit SizeEach

Contenido y almacenamiento

Contains Lysis Reagents (2 -8°C): 20 mL each Lysis and DNA Stabilization Solution; Master Mix (15 to 25°C): 50 mL 2X master mix with AmpliTaq™ Fast DNA Polymerase, UP, dNTPs, Tracking dye and ROX™ Dye.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

Preguntas frecuentes

When designing probes to perform Allelic Discrimination (SNP assays), are there any alternatives to using probes longer than 30bp in order to get the appropriate melting temperature?

Applied Biosystems TaqMan MGB (minor groove binder) probes would be appropriate for Allelic Discrimination/SNP Genotyping probe designs. TaqMan MGB probes have a minor groove binder at the end of the probe. This minor groove binder increases the Tm of probes, allowing the use of shorter probes. Consequently, the TaqMan MGB probes exhibit greater differences in Tm values between matched and mismatched probes, which provides more accurate allele discrimination.

For information on the design of TaqMan MGB probes for Allelic Discrimination/SNP assays using Primer Express software, please refer to our guide "Designing TaqMan MGB Probe and Primer Sets for Allelic Discrimination Assays Using Primer Express".