Applied Biosystems™

TaqMan™ Sample-to-SNP™ Kit (5 mL sample prep, 1 mL PCR)

Catalog number: 4403313
Applied Biosystems™

TaqMan™ Sample-to-SNP™ Kit (5 mL sample prep, 1 mL PCR)

Catalog number: 4403313
Catalog Number
4403313
Unit Size
1 kit
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
44033131 kit
-
Product Overview
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The Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than one hour. The TaqMan™ Sample-to-SNP™ Kit combines DNA extraction and TaqMan™ chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

• DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
• The TaqMan™™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2°–8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems™ TaqMan™ Genotyping Assays
• Extended PCR cycling for poorly amplified or sample-limited reactions

The kit includes reagents sufficient for 100 sample preparation reactions at 50 μL/ reaction and 400 PCR reactions at 5 μL for each reaction.

Please note:
The DNA Extract All reagents are not available for individual sale. They are only available as a part of the TaqMan™ Sample-to-SNP™ Kit.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Primer-probe
GC-Rich PCR Performance
Low
PCR Method
qPCR
Polymerase
AmpliTaq DNA Polymerase
Reaction Speed
Fast or Standard
Technique
SNP Genotyping (Real-Time PCR-Based)
For Use With (Equipment)
7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, GeneAmp 9700, StepOne™, Fast Mode, StepOne™, Standard Mode, Veriti Thermal Cycler, StepOne™ Standard Mode, StepOne™ Fast Mode
Genotyping Target
SNPs (Unknown or Numerous), SNPs (Known)
Passive Reference Dye
ROX (Pre-mixed)
Product Line
Sample-to-SNP™, TaqMan™
Product Type
Sample to SNP Kit
Purity or Quality Grade
UP (Ultra Pure)
Sufficient For
100 Reactions
Hot Start
Built-In Hot Start
Sample Type
Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood
Volume
5 mL

Contents & Storage

Lysis Reagents (2° to 8°C): 5 mL each Lysis and DNA Stabilization Solution; Master Mix (15° to 25°C ): 1 mL 2X master mix with AmpliTaq™ Fast DNA Polymerase, UP, dNTPs, Tracking Dye and ROX™ Dye.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

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