Applied Biosystems™
TaqMan™ Sample-to-SNP™ Kit (5 mL sample prep, 1 mL PCR)
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Description
The Applied Biosystems® TaqMan® Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than one hour. The TaqMan® Sample-to-SNP™ Kit combines DNA extraction and TaqMan® chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.• DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
• The TaqMan®™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2°–8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays
• Extended PCR cycling for poorly amplified or sample-limited reactions
The kit includes reagents sufficient for 100 sample preparation reactions at 50 µL/ reaction and 400 PCR reactions at 5 µL for each reaction.
Please note:
The DNA Extract All reagents are not available for individual sale. They are only available as a part of the TaqMan® Sample-to-SNP™ Kit.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Detection Method: | Primer-Probe Detection |
|---|---|
| For Use With (Equipment): | 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, GeneAmp 9700, StepOne™, Fast Mode, StepOne™, Standard Mode, Veriti Thermal Cycler |
| Genotyping Target: | SNPs (Known), SNPs (Unknown or Numerous) |
| Hot Start: | Built-In Hot Start |
| PCR Method: | qPCR |
| Passive Reference Dye: | ROX (Pre-mixed) |
| Polymerase: | AmpliTaq® DNA Polymerase |
| Primer-Probe Compatibility: | TaqMan® |
| Product Line: | Sample-to-SNP™, TaqMan™ |
| Product Size: | 1 kit |
| Purity or Quality Grade: | UP (Ultra Pure) |
| Reaction Speed: | Fast, Standard |
| Sample Type (General): | Blood, Buccal Samples, Cells, Plant Samples, Tissue |
| Sample Type (Specific): | Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood |
| Technique: | SNP Genotyping (Real-Time PCR-Based) |
Contents & storage
Lysis Reagents (2 -8°C): 5 mL each Lysis and DNA Stabilization Solution; Master Mix (15 to 25°C ): 1 mL 2X master mix with AmpliTaq® Fast DNA Polymerase, UP, dNTPs, Tracking Dye and ROX™ Dye.
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
