Informational:Beginning August 15, 2014, this product will ship under ambient conditions. Full details can be found here.
Applied Biosystems™

TaqMan™ Copy Number Reference Assay, human, RNase P

Green features
Catalog number: 4403328
Applied Biosystems™

TaqMan™ Copy Number Reference Assay, human, RNase P

Green features
Catalog number: 4403328
Catalog Number
4403328
Unit Size
3,000 reactions
Price (USD)
Price: 427.00
Your Price:
Quantity
Catalog Number
Unit Size
750 reactions
Price (USD)
Price: 144.00
Your Price:
Quantity
Catalog NumberUnit SizePrice (USD)Quantity
44033283,000 reactions
Price: 427.00
Your Price:
4403326750 reactions
Price: 144.00
Your Price:
Product Overview
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Additional Information
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Human TaqMan™ Copy Number Reference Assays are run with human TaqMan™ Copy Number Assays in a duplex real-time PCR reaction to detect and measure copy number variations (CNVs) and smaller regions in the human genome. Two options for genes that can be used as endogenous reference genes in humans are offered: RNase P and TERT. TaqMan™ Copy Number Reference Assays are designed to unique genomic sequences in the reference genome assembly (build GRCh38) and are required for relative quantitation of copy number targets.

TaqMan™ Copy Number Assays quantitate the gene of interest and normalize to an endogenous reference gene known to be present in two copies in a diploid genome. Please note that TaqMan™ Copy Number Reference Assays are species specific.

RNase P: The Standard Reference Gene Option
TaqMan™ Copy Number Reference Assays have a VIC™ dye–labeled TAMRA™ probe and reference sequence–specific forward and reverse primers. The assays are not primer-limited.

TaqMan™ Copy Number Reference Assay RNase P is recommended as the standard reference assay for copy number analysis. This assay detects the Ribonuclease P RNA component H1 (H1RNA) gene (RPPH1) on chromosome 14, cytoband 14q11.2. The assay location is chr.14:20343370 on build GRCh38. It has an 87 bp amplicon that maps within the single exon RPPH1 gene.

TaqMan™ Copy Number Reference Assay TERT is an alternative reference assay; it is recommended in the event that the RNase P assay functions poorly with a sample because of chromosomal aberrations or other issues. This assay targets the telomerase reverse transcriptase (TERT) gene located on chromosome 5, cytoband 5p15.33. The assay location is chr5:1253257 on build GRCh38. It has an 88 bp amplicon that maps within exon 16 of the TERT gene.

Simplest Copy Number Analysis Workflow
TaqMan™ Copy Number Assays have the simplest workflow of all currently available copy number analysis methods. The test assay (FAM™ dye–labeled), the reference assay (VIC™ dye–labeled), your sample DNA, and TaqMan™ Master Mix are combined and then run on an Applied Biosystems™ real-time PCR system using the standard TaqMan™ Copy Number Assay protocol. On average, setup to primary analysis takes only 3–4 hr (including an approximately 2 hour PCR run).

Powerful Data analysis Software
CopyCaller™ Software was developed specifically for TaqMan™ Copy Number Assay data analysis. This free, easy-to-use software utilizes a graphical interface that quickly calculates the possible copy numbers for a set of samples in a run. It also gives a confidence value for each copy number call, and has outlier functionality.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

3'Primer Modification
None
5'Primer Modification
None
Concentration
20X
Detection Method
Primer-Probe
Form
Frozen
Format
Tube
GC-Rich PCR Performance
Low
No. of Reactions
3000 Reactions
PCR Method
qPCR
Reaction Speed
Standard
Gene Symbol
RPPH1
Green Features
Sustainable packaging
Includes
Control Primer-Probe Set(s)
Internal Probe Modification
TAMRA™ Quencher (3'), VIC™ (5')
Label or Dye
VIC
Product Line
TaqMan™
Product Type
Configurable Assay
Purification Method
HPLC
Purity or Quality Grade
RNase-Free, DNase-Free
Shipping Condition
Ambient Temperature
Species
Human
Sufficient For
3000 Reactions
Target Gene
RNase P

Contents & Storage

Contains 4 tubes (750 μL each) of 20X pre-formulated assay with reference sequence-specific forward and reverse primers and 1 VIC™ dye-labeled TAMRA™ probe. The tube contains enough assay for 3000 20-μL reactions or 6000 10-μL reactions in 96-well format plates. Assays should be stored at -15°C to -25°C in the dark. Minimize freeze-thaw cycles.