Applied Biosystems™ TaqMan™ Protein Expression Assays enable fast, easy identification, and relative protein quantification of pluripotency protein markers from limited quantities of cultured hESCs. The TaqMan™ Protein Expression Assay Kit (Human ICAM1) contains the assay probes and a dilution buffer for the labelling of human inter-cellular adhesion molecule 1 protein (hICAM1) in cell lysates prepared using the Protein Expression Sample Preparation Kit. The assay probes consist of two oligonucleotides conjugated to antibodies that bind to separate epitopes of the hICAM1 protein. Key features of the TaqMan™ Protein Expression Assay: • Quantitates protein expression using gold standard TaqMan™ 5’ nuclease chemistry • Enables direct correlation of mRNA and/or miRNA expression to protein expression on the same real-time PCR platform • Allows greater sensitivity and a more quantitative readout than western blots and immunostaining
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection Method
Primer-probe
GC-Rich PCR Performance
Low
PCR Method
qPCR
Polymerase
AmpliTaq Fast DNA Polymerase
Reaction Speed
Fast
Technique
Proximity Ligation Assay
For Use With (Equipment)
7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Green Features
Sustainable packaging
Label or Dye
FAM
Passive Reference Dye
ROX (Pre-mixed)
Product Line
TaqMan™
Product Type
Protein Expression Assay Kit
Purity or Quality Grade
UP (Ultra Pure)
Shipping Condition
Wet Ice
Sufficient For
100 Reactions
Target Specificity
Target-Specific
Hot Start
No
No. of Reactions
100 Reactions
Sample Type
Cells
Contents & Storage
Contains Assay Probe A, Assay Probe B, and Assay Probe Dilution Buffer. The assay probes (Assay Probe A and Assay Probe B) are supplied at a 20x concentration. The Assay Probe Dilution Buffer is supplied at a 1x concentration. Store at -15 to -25°C.