Applied Biosystems™

TaqMan™ Protein Expression Assay Kit (hICAM1) This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  4405471

 Related applications:

Real Time PCR (qPCR)

| Stem Cell Analysis

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Applied Biosystems® TaqMan® Protein Expression Assays enable fast, easy identification, and relative protein quantification of pluripotency protein markers from limited quantities of cultured hESCs. The TaqMan® Protein Expression Assay Kit (Human ICAM1) contains the assay probes and a dilution buffer for the labelling of human inter-cellular adhesion molecule 1 protein (hICAM1) in cell lysates prepared using the Protein Expression Sample Preparation Kit. The assay probes consist of two oligonucleotides conjugated to antibodies that bind to separate epitopes of the hICAM1 protein. Key features of the TaqMan® Protein Expression Assay:
• Quantitates protein expression using gold standard TaqMan® 5’ nuclease chemistry
• Enables direct correlation of mRNA and/or miRNA expression to protein expression on the same real-time PCR platform
• Allows greater sensitivity and a more quantitative readout than western blots and immunostaining
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Primer-Probe Detection
For Use With (Equipment): 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Hot Start: No
Label or Dye: FAM
PCR Method: qPCR Protein Quantification
Passive Reference Dye: ROX (Pre-mixed)
Polymerase: AmpliTaq® Fast DNA Polymerase
Primer-Probe Compatibility: TaqMan®
Product Line: TaqMan™
Product Size: 100 reactions
Purity or Quality Grade: UP (Ultra Pure)
Reaction Speed: Fast
Sample Type (General): Cells
Target Specificity: Target-Specific
Technique: Proximity Ligation Assay
Green Features: Sustainable packaging
Shipping Condition: Wet Ice

Contents & storage

Contains Assay Probe A, Assay Probe B, and Assay Probe Dilution Buffer. The assay probes (Assay Probe A and Assay Probe B) are supplied at a 20x concentration. The Assay Probe Dilution Buffer is supplied at a 1x concentration. Store at -15 to -25°C.


Manuals & protocols