Applied Biosystems™

Applied Biosystems™ 7500 Fast Dx Real-Time PCR Instrument, with laptop computer

Catalog number: 4406984
Applied Biosystems™

Applied Biosystems™ 7500 Fast Dx Real-Time PCR Instrument, with laptop computer

Catalog number: 4406984
Catalog Number
4406984
Unit Size
1 system
Price (USD)
Catalog NumberUnit SizePrice (USD)
44069841 system
Product Overview
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The Applied Biosystems® 7500 Fast Dx Real-Time PCR Instrument with SDS Software is a real-time nucleic acid amplification and five-color fluorescence detection system available for in vitro diagnostic use. The 7500 Fast Dx Real-Time PCR Instrument delivers the performance required for high-quality results in a 96-well format.

• 96-well format eases plate setup if automation is not available
• Accommodates tube strips, which can be capped immediately after pipetting each sample
• Fast mode completes runs in less than forty minutes
• Optional standard mode facilitates use of standard length real-time PCR assays without changing thermal cycling parameters
• 5-color variable excitation enables multiplex assays

Convenient 96-well Format
The 7500 Fast Dx Real-Time PCR Instrument minimizes the effort required to perform sample setup while also allowing the use of tube strips.

Intuitive Software to Run Pre-optimized Protocols
The 7500 Fast Dx instrument's Sequence Detection Software Security, Auditing, and E-Signature module offers the flexibility to develop assays and also run pre-optimized protocols for users operating in a secure environment.

Instrument Service and Support
The 7500 Fast Dx Real-Time PCR Instrument is supported by our AB Assurance Service Plan for Diagnostics, which includes:
• Instrument Installation Qualification, Operation Qualification, and Performance Qualification (IQ⁄OQ⁄PQ)
• Spectral calibration and software setup
• Emergency repair, including parts and labor, and OQ⁄PQ after any major part change
• Planned maintenance every 12 months

Europe: For in vitro Diagnostic Use. The 7500 Fast Dx Real-Time PCR Instrument meets the requirements of the in vitro Diagnostic Medical Devices Directive (98⁄79⁄EC). The CE IVD-registered 7500 Fast Dx Real-Time PCR Instrument is for distribution and use in specific European countries only.

USA: For in vitro Diagnostic Use. The 7500 Fast Dx Real-Time PCR instrument is 510(k)-cleared (K082562). The customer is responsible for any validation of assays and compliance with any regulatory requirements that pertain to their procedures and instrument use.

Specifications

For Use With (Equipment)
7500 Fast Dx System
Includes
Laptop Computer
Reaction Speed
Fast, Standard
Dynamic Range
9 logs of linear dynamic range
Sensitivity
Down to 1 copy of human RNAse P gene
Capacity
12 x 8-tube strips, 1 x 96-well plate (Fast)
Calibrated Dye
TAMRA, Cy3, FAM, SYBR Green, Texas Red, JOE, ROX, Cy5, NED, VIC
Dimensions
34 cm (13.39 in) (W) x 49 cm (19.29 in) (H) x 45 cm (17.72 in) (D)
External Computer
Laptop computer provided
Format
96-well plate, 8-well strips
Passive Reference Dye
ROX (Separate Tube), Other Passive Reference Dye, ROX (Pre-mixed)
Optics
CCD Camera, Halogen light source, Five excitation and five emission filters
Peak Block Ramp Rate
5.5°C⁄sec
Precision
Distinguish between 5,000–10,000 genome equivalents (two-fold copy number difference) with 99.7% confidence
Run Time
36 min (Fast), <2 hrs (Standard)
Reaction Volume Range
10-30 µl
Sample Ramp Rate
Fast mode: ± 3.5°C⁄sec, Standard mode: ± 1.6°C⁄sec
Temperature Range (Metric)
4.0-99.9 °C
Temperature Uniformity
±1°C
Temperature Accuracy
±0.5°C
Throughput
4 to 5 96-well plates per day
Thermal Cycling System
Peltier-Based System
Weight
34 kg (75 lb)
Includes
Laptop
Max. Heating Rate (Thermal Block)
5.5°C

Contents & Storage

The package includes one real-time PCR instrument, one Dell laptop computer, Primer Express Software, and Sequence Detection Software. Recommended operating conditions: Temperature: 15 to 30 °C (59 to 86 °F). Maximum change of less than 15 degrees Celsius (27 °F) per 24 hrs. Humidity: 20 to 80% relative humidity, noncondensing.

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Frequently asked questions (FAQs)

Citations & References