The Applied Biosystems® 7500 Fast Dx Real-Time PCR Instrument with SDS Software is a real-time nucleic acid amplification and five-color fluorescence detection system available for in vitro diagnostic use. The 7500 Fast Dx Real-Time PCR Instrument delivers the performance required for high-quality results in a 96-well format.
• 96-well format eases plate setup if automation is not available • Accommodates tube strips, which can be capped immediately after pipetting each sample • Fast mode completes runs in less than forty minutes • Optional standard mode facilitates use of standard length real-time PCR assays without changing thermal cycling parameters • 5-color variable excitation enables multiplex assays
Convenient 96-well Format The 7500 Fast Dx Real-Time PCR Instrument minimizes the effort required to perform sample setup while also allowing the use of tube strips.
Intuitive Software to Run Pre-optimized Protocols The 7500 Fast Dx instrument's Sequence Detection Software Security, Auditing, and E-Signature module offers the flexibility to develop assays and also run pre-optimized protocols for users operating in a secure environment.
Instrument Service and Support The 7500 Fast Dx Real-Time PCR Instrument is supported by our AB Assurance Service Plan for Diagnostics, which includes: • Instrument Installation Qualification, Operation Qualification, and Performance Qualification (IQ⁄OQ⁄PQ) • Spectral calibration and software setup • Emergency repair, including parts and labor, and OQ⁄PQ after any major part change • Planned maintenance every 12 months
Europe: For in vitro Diagnostic Use. The 7500 Fast Dx Real-Time PCR Instrument meets the requirements of the in vitro Diagnostic Medical Devices Directive (98⁄79⁄EC). The CE IVD-registered 7500 Fast Dx Real-Time PCR Instrument is for distribution and use in specific European countries only.
USA: For in vitro Diagnostic Use. The 7500 Fast Dx Real-Time PCR instrument is 510(k)-cleared (K082562). The customer is responsible for any validation of assays and compliance with any regulatory requirements that pertain to their procedures and instrument use.
34 cm (13.39 in) (W) x 49 cm (19.29 in) (H) x 45 cm (17.72 in) (D)
Laptop computer provided
96-well plate, 8-well strips
Passive Reference Dye
ROX (Separate Tube), Other Passive Reference Dye, ROX (Pre-mixed)
CCD Camera, Halogen light source, Five excitation and five emission filters
Peak Block Ramp Rate
Distinguish between 5,000–10,000 genome equivalents (two-fold copy number difference) with 99.7% confidence
36 min (Fast), <2 hrs (Standard)
Reaction Volume Range
Sample Ramp Rate
Fast mode: ± 3.5°C⁄sec, Standard mode: ± 1.6°C⁄sec
Temperature Range (Metric)
4 to 5 96-well plates per day
Thermal Cycling System
34 kg (75 lb)
Max. Heating Rate (Thermal Block)
Contents & Storage
The package includes one real-time PCR instrument, one Dell laptop computer, Primer Express Software, and Sequence Detection Software. Recommended operating conditions: Temperature: 15 to 30 °C (59 to 86 °F). Maximum change of less than 15 degrees Celsius (27 °F) per 24 hrs. Humidity: 20 to 80% relative humidity, noncondensing.