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MagMAX™ DNA Multi-Sample Kit - FAQs

MagMAX™ DNA Multi-Sample Kit - FAQs

View additional product information for MagMAX™ DNA Multi-Sample Kit - FAQs (4413020)

8 product FAQs found

I'm using your MagMAX DNA Multi-Sample Isolation Kit for isolation of genomic DNA, but I've run out of DNA binding beads. Can I order them as a standalone product?

Yes, please order Cat. No. 4489112.

What is the difference between Proteinase K for MagMAX DNA Multi-Sample Ultra Kit (Cat. No A25561) and Proteinase K for MagMAX 96 DNA Multi-Sample Kit (Cat. No. 4489110)?

The difference between Proteinase K for MagMAX‐96 DNA Multi‐Sample Kit and Proteinase K for MagMAX DNA Multi‐Sample Ultra Kit is the brand. The former is from Invitrogen legacy and the latter is from Applied Biosystems legacy. Both products have an identical concentration of 100 mg/mL and can be used interchangeably.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center

I am using the MagMAX DNA Multi-Sample kit on my MagMAX Express-96 instrument and notice that I start with 100 microliters elution volume and end up with only 80 microliters. How can I prevent loss of the elution volume?

The MagMAX beads will cause a loss of ~2-3 µL of solution when they are pulled from the solution, but this is a very small amount. Larger volume loss, such as 20µL, can be due to several factors:
• Low lab humidity combined with a heated elution can cause this amount of liquid to evaporate. In this case, we recommend increasing the starting elution volume. Since the nucleic acid is not evaporating this should not affect your overall yield.
• Issues with alignment of the instrument components could also result in loss of volume. If the head goes down too deep during mixing, it could pull droplets up along the side. This would reduce elution volume and you would also be more likely to see a reduction in yield. A similar issue would result if the turntable alignment was off on either side.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

Can I edit the manufactured KingFisher Flex protocols?

No, manufactured protocols are locked for editing, but they can be renamed using the ‘save as' function and edited as a new version.

Can I use magnetic beads from other vendors with the KingFisher Flex instrument?

For nucleic acid extraction, we recommend using our MagMAX kits and MagJET kits. Other magnetic beads should work well as long as they are 0.5 to 10 µm in size. However, you will have to write your own KingFisher protocols using the Thermo Scientific BindIt software and validate/optimize the protocol on your own.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

I am working with liquid samples with high volume and want to extract nucleic acid from them using the KingFisher Flex instrument. Can I use more volume than the protocol advises?

Yes, this can be done by sample splitting. Set the protocol so that the magnet will collect samples from multiple wells before continuing to the next step. Be sure not to overload.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

Can I scale up my reaction volume with the KingFisher Flex instrument?

Yes, reaction volumes can be scaled up, and all other volumes (i.e. wash volumes) will have to be increased by the same fold difference. It is generally not recommended to scale up reaction volumes more than 2-fold unless the plate's volume capacity will allow it.

Are all of the Applied Biosystems MagMAX kits validated for the KingFisher Flex Magnetic Particle Processor?

Yes, all MagMAX kits can be used with the KingFisher Flex Magnetic Particle Processor.