AmpFLSTR® NGM™ PCR Amplification Kit

Catalog number: 4415020

Applied Biosystems™  Related applications: PCR Amplification for Human Identification

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Description

The NGM™ Kit is a highly robust Short Tandem Repeat (STR) multiplex kit which utilizes a single protocol to amplify the 10 SGM Plus™ loci (D2S1338, D3S1358, D8S1179, D16S539, D18S51, D19S433, D21S11, FGA, TH01, vWA, Amelogenin) together with the additional loci approved by the European Union Council for the expansion of the European Standard Set (D10S1248, D22S1045, D2S441, D1S1656 & D12S391). The kit combines the latest advances in buffer optimization, primer synthesis and thermal cycling optimization to deliver high sensitivity and improved STR performance for forensic casework and database samples in one easy workflow.

Key Features of the NGM™ Kit:

• Simultaneous amplification of the SGM Plus® kit loci plus 5 additional ENFSI/EDNAP recommended loci to provide powerful discrimination and facilitate cross-border data sharing
• Unprecedented performance in the presence of significant inhibitors combined with enhanced profile and locus balance
• Increased ability to recover information from degraded samples through a concentration of highly discriminatory loci in the low molecular weight region
• Greater sensitivity for improved amplification of low-level samples and better detection of the minor contributor in mixed samples
• Exceptional profile quality and clean baseline resulting from improved primer synthesis and purification processes
• 100% concordance with previously typed samples through maintenance of all STR primer sequences for loci common to the SGM Plus®, Identifiler® and SEfiler Plus™ Kits
• Proprietary and proven mobility modifier technology enables optimal locus spacing
• One kit, one protocol delivers high quality results from both database and casework samples
• Streamlined workflow with reduced amplification time, enzyme pre-formulated within the master mix and a larger package size option (1000 reactions) for high-throughput laboratories
• Optimized as part of an integrated reagent, instrumentation and software system for maximum performance and efficiency
• Outstanding discrimination and robustness offers utility for specialized applications including complex paternity/kinship, disaster victim identification and missing persons cases

Note: For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

“This reagent is classified as a medical device in several countries. If it is not regulated as a medical device in specific region, it should be used for laboratory use only.”
For Forensic or Paternity Use Only.

Specifications

Technique: STR (Short Tandem Repeat) Analysis
Polymerase: AmpliTaq Gold® DNA Polymerase
Label or Dye: NED™, PET®, LIZ®, VIC®, FAM™
Product Size: 200 reactions
Amplicon Size: <380bp
Sample Volume: 25 µl⁄reaction
Reaction Format: SuperMix or Master Mix
Sample Type (Specific): DNA (Genomic), Forensic Sample
For Use With (Equipment): GeneAmp® 9700, 3730 DNA Analyzer, 3130xl Genetic Analyzer, 310 Genetic Analyzer, 3100-Avant™ Genetic Analyzer, 3130 Genetic Analyzer, 3500 Genetic Analyzer, GeneAmp® 9600, 3500xL Genetic Analyzer
Matrix and Size Standards: Matrix Standard Set DS-33, GeneScan™ 500 LIZ® Size Standard
Shipping Condition: Dry Ice

Contents & storage

• AmpFLSTR® NGM™ Master Mix
• AmpFLSTR® NGM™ Primer Set
• AmpFLSTR® NGM™ Allelic Ladder
Store at ?15 to ?25 °C upon receipt, 2 to 8 °C after initial use

• AmpFLSTR® Control DNA 007
Store at 2 to 8 °C

Documents

Manuals & protocols