The USDA licensed, VetMAX™ -Gold SIV Detection Kit is a robust PCR diagnostic kit, enabling early detection of swine influenza virus in pigs and helping to minimize the spread of the disease.
Licensed by the USDA
• Targets multiple genomic regions, reducing the risk of false negatives caused by genomic mutation • Enables unsurpassed sensitivity, specificity, repeatability, and reproducibility • Decreases variability in results and allows for reproducibility through assay standardization
The VetMAX™-Gold SIV Detection Kit is the first and only USDA-licensed real-time PCR test for the rapid detection of SIV RNA purified from porcine nasal swab samples. This highly sensitive, qualitative, one-step assay delivers accurate results within 1.5 hours. The VetMAX™-Gold SIV Detection assay is a single-tube (or well) real-time RT-PCR assay in which SIV and Xeno™ internal positive control RNA is reverse transcribed into cDNA, then amplified and detected in real time using fluorescent TaqMan™ probes.
Confidence in Results The VetMAX™-Gold SIV Detection Kit utilizes AmpliTaq Gold™ DNA Polymerase and optimized primers and TaqMan™ probes for maximum sensitivity and specificity. Additionally, the included Xeno™ RNA internal positive control enables monitoring of RNA purification efficiency and PCR inhibition, allowing for increased accuracy.
Designed to fit within a simple streamlined workflow, the VetMAX™-Gold SIV Detection Kit is optimized to perform with the MagMAX™ Pathogen RNA/DNA Kit and the Applied Biosystems™ 7500 Fast Real-Time PCR Systems.
Note: This kit is not intended for the differentiation of SIV subtypes.
仕様
検出法Primer-Probe Detection
使用対象(アプリケーション)Sequencing, Real-Time PCR
反応数100
PCR法1-Step qRT-PCR
製品ラインVetMAX
製品タイプGold SIV Detection Kit
数量100 reactions
サンプルタイプSwab
出荷条件Dry Ice
種Swine/Porcine
対象微生物クラスSwine Influenza Virus (SIV)
フォーマットキット
Unit SizeEach
組成および保存条件
Store at -30°C to -10°C; Includes 100 reactions with the following components:
• Multiplex RT-PCR buffer • Multiplex enzyme mix • Nuclease-free water • SIV primer-probe mix • SIV-Xeno RNA control mix • XenoRNA control
よくあるご質問(FAQ)
What are the quenchers in the VetMAX-Gold SIV Detection Kit?
The VetMAX-Gold SIV Detection Kit contains the following quenchers:
- SIV RNA: Eclipse® Q
- Xeno RNA Control: Eclipse® Q
What are the assay-labeled reporters in the VetMAX-Gold SIV Detection Kit?
The VetMAX-Gold SIV Detection Kit contains the following assay-labeled reporters:
- SIV RNA: FAM dye
- Xeno RNA Control: VIC dye
What is the internal positive control (IPC) in the VetMAX-Gold SIV Detection Kit?
The VetMAX-Gold SIV Detection Kit contains the Xeno RNA Control as the internal positive control (IPC) for the RNA purification process and to monitor for the presence of PCR inhibitors.