Applied Biosystems™

MeltDoctor™ HRM Reagent Kit

Catalog number:  4425557

 Related applications:

Genotyping & Genomic Profiling

| Methylation Analysis

| Real Time PCR (qPCR)

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Applied Biosystems® MeltDoctor™ HRM Reagent Kit provides individually packaged PCR components and the MeltDoctor™ HRM Dye required for high resolution melting analysis. High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences.
This kit includes:
• AmpliTaq Gold® 360 DNA Polymerase which, when combined with AmpliTaq Gold® 360 Buffer and 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. AmpliTaq Gold® 360 DNA Polymerase delivers 360° coverage for a full range of targets.
• GeneAmp® dNTP Blend
• MeltDoctor™ HRM Dye, a stabilized form of the fluorescent SYTO® 9 double-stranded nucleic acid stain developed by Molecular Probes
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Fluorescent
For Use With (Equipment): 7500 Fast System, 7900HT Fast System
Genotyping Target: Epigenetic Targets, SNPs (Unknown or Numerous)
Label or Dye: HRM (High Resolution Melting) Dye, SYTO® 9
Polymerase: AmpliTaq Gold 360 DNA Polymerase
Product Line: MeltDoctor™
Product Size: 1 kit
Sample Type (General): DNA
Sample Type (Specific): DNA (Genomic), cDNA
Technique: HRM (High Resolution Melting) Analysis, Mutation Scanning

Contents & storage

This kit includes: AmpliTaq Gold® 360 DNA Polymerase (2 x 50 µL, each at 5 U/µL), AmpliTaq Gold® 360 Buffer, 360 GC Enhancer, GeneAmp® dNTP Blend, and MeltDoctor™ HRM Dye (20x). The kit provides sufficient materials for 250 20-µL reactions when using 2 U AmpliTaq Gold 360 DNA Polymerase per reaction.

Store at -15° to -25°

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).


Manuals & protocols