MeltDoctor™ HRM Calibration Plate, Fast 96-well

Las placas de calibración MeltDoctor® HRM listas para su uso Applied Biosystems™ contienen todos los componentes necesarios para el coloranteMás información

Número de catálogo	Cantidad
4425618	1 x 96-well plate

Número de catálogo 4425618

Precio (MXN)

Cantidad:

1 x 96-well plate

Las placas de calibración MeltDoctor® HRM listas para su uso Applied Biosystems™ contienen todos los componentes necesarios para el colorante puro y la calibración de HRM para reducir la complejidad de comenzar con la experimentación de HRM.Estas placas proporcionan la calibración térmica y óptica necesaria para una discriminación precisa y reproducible entre las curvas de fusión.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Tinte calibradoColorante de HRM (fusión de alta resolución)

Tipo de calibraciónCalibración térmica, calibración óptica

Para utilizar con (aplicación)PCR en tiempo real (PCRq)

Para utilizar con (equipo)7500 sistema Fast, 7900HT sistema Fast

FormatoPlaca de 96 pocillos

Compatibilidad de placasPlaca de calibración

Línea de productosMeltDoctor

Cantidad1 x 96-well plate

Velocidad de reacciónRapidez

Condiciones de envíoHielo seco

Tipo de productoPlaca de calibración de HRM

Unit SizeEach

Contenido y almacenamiento

1 placa, formato de 96 pocillos Fast

La vida útil mínima garantizada es de 60 días (fecha de caducidad exacta impresa en el producto y en el certificado de análisis).

Preguntas frecuentes

How long should the amplicon be for HRM analysis?

We recommend to use 50-250 bp long PCR amplicons. Typically, shorter amplicons can distinguish the genotypes for a SNP better, especially for Type III and Type IV SNPs. This is simply because a single base variation affects the melting behavior of a 100 bp amplicon stronger than of a 500 bp amplicon, for example. In longer fragments, the risk of covering multiple mutations is also increased.

What can be used as positive control reactions in HRM analysis?

Positive control reactions should contain template DNA with a known sequence. In SNP genotyping experiments, this could be a sample with a known genotype. Positive control(s) for all genotypes should be included where possible to serve as a reference in melting curve comparison and assigning genotypes for test samples. In mutation scanning experiments, a sample with a wild type sequence could serve as a positive control. The controls should preferably have the same DNA concentration as their corresponding test samples. Control DNA should also be eluted and/or diluted in the same buffer as the samples.

In HRM analysis, when is it better to use a 3-step cycling protocol rather than a 2-step protocol?

A 3-step cycling protocol is recommended for the analysis of complicated (especially Type IV SNP) targets, amplicons longer than 200 bp, and amplicons with a primer annealing temperature that is less than 60 degrees C.

The HRM software will not accept my calibration file. What is wrong?

Check that there are no outliers on the plate. You cannot omit any wells on the HRM calibration plate.

I have a 7900HT Fast Real-Time PCR System, and cannot open the data file in HRM software. What could be causing this?

There are a few possibilities. First, make sure the HRM Software version is v2.0.1, and the 7900HT Fast Real-Time PCR System software version is v2.3 or above. Second, check that the run method used was as recommended in the HRM protocol; make sure the ramp rate for the dissociation stage is 1%. Then try to open the calibration file from the HRM Software; if it does not open, the calibration file is defective. The defects could be due to a bad calibration plate or instrument uniformity issue.

View all MeltDoctor™ HRM Calibration Plate, Fast 96-well FAQs