Applied Biosystems™

TaqMan™ Pri-miRNA Assay

Green features
Catalog number: 4427012
Applied Biosystems™

TaqMan™ Pri-miRNA Assay

Green features
Catalog number: 4427012
Catalog Number
4427012
Unit Size
S (360 reactions), made to order
Price (USD)
361.00
Your Price:
Catalog Number
Unit Size
M (750 reactions), made to order
Price (USD)
658.00
Your Price:
Catalog Number
Unit Size
L (2900 reactions), made to order
Price (USD)
1,298.00
Your Price:
Catalog NumberUnit SizePrice (USD)
4427012S (360 reactions), made to order
361.00
Your Price:
4427013M (750 reactions), made to order
658.00
Your Price:
4427014L (2900 reactions), made to order
1,298.00
Your Price:
Product Overview
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Additional Information
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TaqMan Pri-miRNA Assays quantitate primary microRNA (pri-miRNA) transcripts, the most upstream RNA molecules in the microRNA biogenesis pathway. They are designed using our state-of-the-art bioinformatics pipeline, delivering gold-standard assay performance and data quality for pri-miRNA studies.

Highly specific—quantitiate miRNA transcription from a single genomic locus
Fast, simple, and scalable—two-step RT-qPCR provides high-quality results
A new perspective—measure miRNA expression at the gene level

TaqMan Pri-miRNA Assays apply the same high sensitivity, superior specificity, and wide linear dynamic range for which all TaqMan genomic assays are renowned to the detection of pri-miRNA transcripts. As a result of their high sensitivity, TaqMan Pri-miRNA Assays require as little as 1 ng of sample input to quantify expression from moderate- to high-expression miRNA loci.

Design and selection
Since all pri-miRNA transcripts have not been mapped, these assays are designed within close proximity to each stem-loop sequence represented in the Sanger miRBase sequence repository. This ensures accurate measurement of the transcript of interest. Each publicly available stem-loop can be matched to a TaqMan Pri-miRNA Assay and TaqMan MicroRNA Assay, enabling the RNA sequences produced at either end of the miRNA maturation pathway to be quantified independently.

Pre-formulated assay (20X; 60X mix)
• 2 unlabeled PCR primers (900 nM each final 1X reaction concentration; 150 nM final 1X reaction concentration)
• 1 FAM dye-labeled TaqMan MGB probe (250 nM final 1X reaction concentration)

For Research Use Only. Not for use in diagnostic procedures.

Specifications

CTA Type
Magellan_PDP_OrderNow
Generic Product
TaqMan miRNA
Green Features
Sustainable packaging
No. of Tests
360 Reactions
Product Line
TaqMan™
Product Type
Pri-miRNA Assay
Shipping Condition
Room Temperature
Sufficient For
360 Reactions
Concentration
60 X
Fidelity (vs. Taq)
60 X
Format
Tube
GC-Rich PCR Performance
High
No. of Reactions
360 rxn(s)
PCR Method
RT-qPCR
Reaction Speed
Fast

Contents & Storage

1 tube containing a 20X (S and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.

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Frequently asked questions (FAQs)

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