TaqMan Pri-miRNA Assays quantitate primary microRNA (pri-miRNA) transcripts, the most upstream RNA molecules in the microRNA biogenesis pathway. They are designed using our state-of-the-art bioinformatics pipeline, delivering gold-standard assay performance and data quality for pri-miRNA studies.
• Highly specific—quantitiate miRNA transcription from a single genomic locus • Fast, simple, and scalable—two-step RT-qPCR provides high-quality results • A new perspective—measure miRNA expression at the gene level
TaqMan Pri-miRNA Assays apply the same high sensitivity, superior specificity, and wide linear dynamic range for which all TaqMan genomic assays are renowned to the detection of pri-miRNA transcripts. As a result of their high sensitivity, TaqMan Pri-miRNA Assays require as little as 1 ng of sample input to quantify expression from moderate- to high-expression miRNA loci.
Design and selection Since all pri-miRNA transcripts have not been mapped, these assays are designed within close proximity to each stem-loop sequence represented in the Sanger miRBase sequence repository. This ensures accurate measurement of the transcript of interest. Each publicly available stem-loop can be matched to a TaqMan Pri-miRNA Assay and TaqMan MicroRNA Assay, enabling the RNA sequences produced at either end of the miRNA maturation pathway to be quantified independently.
Pre-formulated assay (20X; 60X mix) • 2 unlabeled PCR primers (900 nM each final 1X reaction concentration; 150 nM final 1X reaction concentration) • 1 FAM dye-labeled TaqMan MGB probe (250 nM final 1X reaction concentration)
For Research Use Only. Not for use in diagnostic procedures.
S (360 reactions), made to order
Contents & storage
1 tube containing a 20X (S and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).