Citations & References (133)

Invitrogen™

Human Silencer Select siRNA INV IN PLATES

Name: Human Silencer Select siRNA INV IN PLATES
SKU: 4427030

Catalog number 4427030

Price (MXN)

Specifications

Unit Size0.1 nmol

Frequently asked questions (FAQs)

Do you offer any siRNA libraries?

Yes, we have a pre-plated collection available for the entire human genome and for different functional classes as Silencer Select Libraries. We also offer custom Silencer Select library services and/or Silencer Libraries for human and mouse.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

What do I do if I would like to order a fourth design of Silencer Select?

If you still would like to have 4 siRNAs in your library instead of 3, you have the option of adding 1 siRNA from our Silencer designs for the human library.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

Why do your Silencer Select siRNA Libraries include 3 siRNAs, rather than 4 or more siRNAs per target?

It is generally agreed that you need at least two effective siRNA per target to confirm that a phenotype observed is due to knocking down the intended gene and not due to an off-target effect. Due to the strength of the Silencer Select siRNA design algorithm and the novel chemical modification, which serve to reduce off-target effects without negatively impacting siRNA potency, a significantly higher percentage of Silencer Select siRNAs are effective at eliciting on-target phenotypes as compared to other siRNA technologies.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

What is the typical turnaround time for an Invitrogen siRNA library?

Turnaround times vary depending on the number of siRNAs and the complexity of the plating request. Most Invitrogen siRNA library orders are completed within 1-3 weeks of order processing. An estimated turnaround time for your particular library will be provided with your price quote. Please contact your local sales representative or email us at rnailibraries@lifetech.com for more information or a quote.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

How should I have the siRNAs in my Invitrogen Custom siRNA Library plated?

For custom siRNA libraries, we can provide a number of plating options. Our most requested format is to have the last 1 or 2 columns empty of each 96-well plate, and to have different siRNAs to the same targets plated in separate plates in the same well location. The empty columns facilitate easy inclusion of any desired control in the final transfection plates. When different siRNAs to the same targets are plated in separate plates in the same well location, the siRNAs can easily be pooled with robotics or a multichannel pipettor. In addition, this type of format facilitates analysis by qRT-PCR.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

View all Human Silencer Select siRNA INV IN PLATES FAQs

Citations & References (133)

Citations & References

Abstract

Expression of CYP4A1 in U251 human glioma cell induces hyperproliferative phenotype in vitro and rapidly growing tumors in vivo.

Authors:Guo AM, Sheng J, Scicli GM, Arbab AS, Lehman NL, Edwards PA, Falck JR, Roman RJ, Scicli AG

Journal:J Pharmacol Exp Ther

PubMed ID:18591218

Exogenous 20-hydroxyeicosatetraenoic acid (20-HETE) increases the growth of human glioma cells in vitro. However, glioma cells in culture show negligible 20-HETE synthesis. We examined whether inducing the expression of a 20-HETE synthase in a human glioma U251 cell line would increase proliferation. U251 cells transfected with CYP4A1 cDNA (termed ... More

S100A16, a novel calcium-binding protein of the EF-hand superfamily.

Authors:Sturchler E, Cox JA, Durussel I, Weibel M, Heizmann CW

Journal:J Biol Chem

PubMed ID:17030513

S100A16 protein is a new and unique member of the EF-hand Ca(2+)-binding proteins. S100 proteins are cell- and tissue-specific and are involved in many intra- and extracellular processes through interacting with specific target proteins. In the central nervous system S100 proteins are implicated in cell proliferation, differentiation, migration, and apoptosis ... More

alpha(v)beta(3) Integrin-mediated drug resistance in human laryngeal carcinoma cells is caused by glutathione-dependent elimination of drug-induced reactive oxidative species.

Authors:Brozovic A, Majhen D, Roje V, Mikac N, Jakopec S, Fritz G, Osmak M, Ambriovic-Ristov A

Journal:Mol Pharmacol

PubMed ID:18441044

As a model for determination of the role of integrins in drug resistance, we used alpha(v)beta(3) integrin-negative human laryngeal carcinoma cell line (HEp2) and three HEp2-derived cell clones with a gradual increase of alpha(v)beta(3) integrin expression. The alpha(v)beta(3) integrin expression protects cells from cisplatin, mitomycin C, and doxorubicin. ... More

Transforming growth factor-beta-stimulated endocardial cell transformation is dependent on Par6c regulation of RhoA.

Authors:Townsend TA, Wrana JL, Davis GE, Barnett JV

Journal:J Biol Chem

PubMed ID:18343818

Valvular heart disease due to congenital abnormalities or pathology is a major cause of mortality and morbidity. Understanding the cellular processes and molecules that regulate valve formation and remodeling is required to develop effective therapies. In the developing heart, epithelial-mesenchymal transformation (EMT) in a subpopulation of endocardial cells in the ... More

IL-10 inhibits cysteinyl leukotriene-induced activation of human monocytes and monocyte-derived dendritic cells.

Authors:Woszczek G, Chen LY, Nagineni S, Shelhamer JH

Journal:J Immunol

PubMed ID:18490762

The immunoregulatory cytokine IL-10 plays an essential role in down-modulating adaptive and innate immune responses leading to chronic inflammatory diseases. In contrast, cysteinyl leukotrienes (cysLTs), important proinflammatory mediators of cell trafficking and innate immune responses, are thought to enhance immune reactions in the pathogenesis of diseases, such as bronchial asthma, ... More

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