Path-ID™ Multiplex One-Step Kit

Catalog number: 4440022

Applied Biosystems™  Related applications: Real Time PCR (qPCR)

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The Path-ID™ Multiplex One-Step RT-PCR Kit is designed for multiplex, quantitative, reverse transcription PCR (qRT-PCR). It is optimized for the amplification of up to four targets simultaneously using your RNA samples and TaqMan® primer⁄probe sets. Reactions are run using a single-tube, one-step procedure to reverse-transcribe the RNA and amplify your targets. The kit contains a Multiplex Enzyme Mix with Arrayscript™ reverse transcriptase and AmpliTaq Gold® DNA Polymerase, and a buffer mix.
For Research Use Only. Not for use in diagnostic procedures.


PCR Method: 1-Step qRT-PCR
Reverse Transcriptase: ArrayScript™ Reverse Transcriptase
Polymerase: AmpliTaq Gold® DNA Polymerase
Detection Method: Primer-Probe Detection
Primer-Probe Compatibility: TaqMan®
Reaction Speed: Standard
For Use With (Equipment): StepOne™, Standard Mode, StepOnePlus™, Standard Mode, 7000 System, 7300 System, 7700 System, 7500 Fast System, 7500 System, 7900HT System
Sample Type (General): RNA
Passive Reference Dye: ROX (Pre-mixed)
Form: Liquid
Format: Tube(s)
Hot Start: Built-In Hot Start
Product Size: 1,000 reactions
Sample Type (Specific): mRNA, Total RNA, Viral RNA

Contents & storage

• 14 mL of 2X Multiplex RT-PCR Buffer
• 2 x 1.4 mL of 10X Multiplex Enzyme Mix including AmpliTaq Gold and ArrayScript
• 25 mL of Nuclease-free water
• Store the kit at -20C; the Nuclease-free water may be stored at -20C, 4C, or room temperature

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).


Manuals & protocols