Applied Biosystems™

3500 Genetic Analyzer for Sequence Typing & Fragment Analysis

Catalog number:  4440470

 Related applications: DNA Sequencing | Fragment Analysis | Genotyping & Genomic Profiling | Sanger Sequencing

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


This 8-capillary Genetic Analyzer is specifically configured with software for both fragment analysis and sequence typing applications.

We set a new standard in capillary electrophoresis with the 8-capillary 3500 Genetic Analyzer. These instruments are specifically designed to support the demanding performance needs of validated and process controlled environments, while retaining the unsurpassed application versatility that life science researchers expect from us.

• An 8 capillary system that can easily be upgraded to a 24-capillary system when you’re ready.
• New single-line 505 nm, solid-state long-life laser – utilizes a standard power supply; requires no heat-removal ducting.
• Powerful, integrated data collection and primary analysis software provides real-time assessment of data quality.
• Radio Frequency Identification (RFID) technology tracks key consumables data and records administrative information.
• Advanced multiplexing capabilities for DNA fragment analysis with up to six unique dyes.
• Unrivaled application flexibility – one array and one polymer are used for most applications.
• Simple setup, operation and maintenance – the easiest-to-run, easiest-to-own DNA sequencer to date.

Accurate Reliable Data Quality
The 3500 series systems significantly improves signal uniformity from instrument-to-instrument, run-to-run, and capillary-to-capillary. With a combination of intelligent hardware, powerful new algorithm and reagent combinations, these methods provide major reduction in the range of signal peak heights obtained across multiple 3500 series instruments (see figure 1).

Improved Sizing Precision
New thermal sub-system design gives enhanced performance in demanding fragment applications enabling improved sizing precision versus previous generations of capillary electrophoresis platforms (see figure 2).

Ready to Use Consumables With RFID Tags
The 3500 series introduces ready-to-use consumables with pre-packaged polymer pouches, cathode and anode buffer containers and easy-to-install capillary arrays. Each of these consumables is integrated with RFID tags that enable viewing, tracking and reporting of critical information about reagents and consumables including usage, lot number, part number, expiry date and on-instrument lifetime within the 3500 Series Data Collection Software (see figures 3 and 4).

Easy to Use New Data Collection Software
3500 Series Data Collection software breaks new ground in user-friendly navigation with an intuitive dashboard design, highly visible buttons for common operations, easy-to-read graphical displays to monitor the state of consumables and handy maintenance scheduling calendar functionality. New functionality includes simplified plate set-up, built-in primary analysis with quality control, so you can make decisions about the quality of data as it is produced on the instrument without the need to transfer output files into secondary analysis software packages (see figures 4 & 5).

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.


Analysis Software: GeneMapper® Software, Sequencing Analysis Software, Variant Reporter® Software
Capillary Length: 36 cm, 50 cm
Compatible Polymers: POP-4™, POP-6™, POP-7™
Compatible Products: 3500 Genetic Analyzer Capillary Array (36 cm), 3500 Genetic Analyzer Capillary Array (50 cm), POP-4™ Polymer for 3500⁄3500xL Genetic Analyzers, POP-6™ Polymer for 3500⁄3500xL Genetic Analyzers, POP-7™ Polymer for 3500⁄3500xL Genetic Analyzers
Current Limit: 15 amps
External Computer: Hard Drive: 2x 500GB SATA 3.0 Gb/s and 8 MB Data Burst Cache, Memory: 16 GB (2x 8GB) 1600 MHz DDR3 Non-ECC. 4th Gen Intel Core I7 Processor- 3.1 GHz Turbo Processor, Operating System: Windows 7 SP1
For Use With (Equipment): 3500 Genetic Analyzer
Format: 8-well strips, 96-well plate
Number of Capillaries: 8 Capillaries
Operating Environment: Humidity: 20%-80% (non-condensing), Room temp: <2° fluctuation during runs, Temperature: 15°C-30°C
Operating System: Windows 7 (Service Pack 1)
Plan Duration: 1 Year Limited Warranty on Parts and Labor, Service Installation and Application Training Included
Polymer Consumption: Replace at earliest of 7 days⁄384 samples⁄60 Injections (384-sample pouches), Replace at earliest of 7 days⁄960 samples⁄120 Injections (960-sample pouches)
Product Size: 1 system
Read Length: ≥850 bp using a 50 cm capillary and POP-7™
Throughput: Up to 424 samples per day (using run module HID36 POP-7)
Voltage Limit: Electrophoresis Voltage - Up to 20 kV
Weight: 82 kg (approximately)

Contents & storage

This package consists of the 8 capillary instrument, the 3500 Data Collection Software, a Dell Workstation and monitor as well as reagent kits for system qualification. This package also includes GeneMapper® software for fragment analysis, and software for sequence typing applications.


Manuals & protocols