PrepFiler Express BTA™ Forensic DNA Extraction Kit

Catalog number: 4441351

Applied Biosystems™  Related applications: DNA Extraction | Forensics

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This kit is for use with the AutoMate Express™ Forensic DNA Extraction System. It was designed specifically for challenging samples such as bones, teeth and adhesive based samples such as cigarette butts and tape lifts. We have designed the PrepFiler® chemistry specifically to improve the quantity and quality of DNA isolated from forensic samples, thereby increasing the potential to obtain maximum information from downstream Short Tandem Repeat (STR) analysis.

Key Product Features:
• Designed to improve the yield and overall purity of DNA isolated from both routine and challenging forensic samples.
• Provides high quality DNA, free of PCR inhibitors and suitable for downstream applications such as quantitative real-time PCR and short tandem repeat (STR) analysis
• Simple and fast separation of substrate from lysate with the unique PrepFiler® LySep™ Column
• Quick and easy run setup with ready-to-use pre-filled cartridges
• Closed system that minimizes the risk of contamination and transposition errors
• Processes 1 to 13 samples in a single run
• Rapid implementation with pre-validated, pre-programmed instrument protocols
• Integrated solution including novel reagents, consumables and automation to provide time saving, cost effective and reliable DNA extraction
• Improves downstream genotyping (STR) success rate and profile quality

Streamlined Off-Line Lysis
The PrepFiler Express™ Kits include the innovative PrepFiler LySep™ Column, which dramatically streamlines the off-line lysis portion of the extraction method. PrepFiler™ Lysis buffer or PrepFiler BTA™ Lysis buffer is added directly into the PrepFiler® LySep™ Column along with the sample for incubation. Next, the PrepFiler® LySep™ Column is quickly centrifuged which permits lysate flow through the burstable membrane while the substrate remains behind in the column. The sample tube is then simply ready to be placed on the AutoMate Express™ instrument. This eliminates the manual lysate and substrate transfer steps, saving time and minimizing cross contamination and sample transposition events.

For Research, Forensic, or Paternity Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research, Forensic, or Paternity Use Only. Not for use in diagnostic procedures.


Sample Type (General): Liquid Samples (e.g. Serum), Tissue, Forensic Samples, Blood, Buccal Samples
Sample Type (Specific): Bone, Gum, Cigarette Butts, Tape Lifts, Teeth
Isolation Technology: Magnetic Bead
Downstream Application: PCR, Real-Time Quantitative PCR (qPCR), Forensic Testing
Final Product: Genomic DNA
For Use With (Equipment): AutoMate Express™ Forensic DNA Extraction System
High Throughput Compatibility: Automated Protocols
Product Size: 52 preps
Shipping Condition: Room Temperature
Starting Material (Amount): Varies depending on sample type

Contents & storage

This kit includes BTA Lysis Buffer, LySep™ Columns, AutoMate Express™ tips and a tip holder, PrepFiler™ Bone and Tooth Lysate Tubes, Proteinase K, PrepFiler Express™ cartridges, sample tubes, and elution tubes. The kit may be stored at 18 to 25°C.


Manuals & protocols