Informational:Informational:To receive future technical notifications regarding this product, please click HERE to register.
Applied Biosystems™

PrepSEQ™ 1-2-3 Mycoplasma Nucleic Acid Extraction Kit

Catalog number: 4443789
Applied Biosystems™

PrepSEQ™ 1-2-3 Mycoplasma Nucleic Acid Extraction Kit

Catalog number: 4443789
Catalog Number
4443789
Unit Size
100 reactions
Price (USD)
970.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
4443789100 reactions
970.00
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
Recommendations
The Applied Biosystems PrepSEQ 1-2-3 Nucleic Acid Extraction Kit rapidly extracts Mycoplasma genomic DNA from 100 µL sample volumes of research cell lines, stem cell lines, and cell and tissue therapy samples. In the PrepSEQ 1-2-3 kit protocol, a rapid procedure recovers DNA from total lysate of the test sample using magnetic-bead based technology. The PrepSEQ 1-2-3 kit is optimized for use with the MycoSEQ Mycoplasma Detection Kit.

Features of the PrepSEQ 1-2-3 Nucleic Acid Extraction Kit include:
- High recovery of Mycoplasma genomic DNA from mammalian cell culture samples
- Rapid easy-to-use protocol with results in under 2 hours
- Compatible with the MycoSEQ Mycoplasma Detection assay
- Protocol available for automated sample preparation

High DNA recovery sample preparation
Optimized for use with the MycoSEQ Mycoplasma Detection Kit, the PrepSEQ 1-2-3 Nucleic Acid Extraction Kit uses proprietary magnetic bead-based separation technology to extract Mycoplasma DNA from mammalian cell lines. The PrepSEQ 1-2-3 kit extracts Mycoplasma genomic DNA from research cell lines, stem cell lines, and cellular and tissue therapy samples with high efficiency.

The protocol is recommended for use with a starting sample volume of 100 µL with a maximum cell density of 106 cells. The PrepSEQ 1-2-3 kit provides an easy to use procedure consisting of 1) RNase treatment, 2) proteinase treatment, and 3) DNA extraction using the magnetic bead technology (figure below).

The kit isolates Mycoplasma genomic DNA with high sensitivity from total lysate of test sample. The PrepSEQ 1-2-3 kit and the MycoSEQ kit allow routine screening of high-value cell lines using an easy, rapid and sensitive protocol. The figure below demonstrates how a sample that was spiked with low levels of Mycoplasma bovoculi DNA was found negative for Mycoplasma contamination using a commercially prepared kit. When using the PrepSEQ 1-2-3 kit and the MycoSeq kit, the correct positive call for Mycoplasma contamination was made.

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Isolation Technology
Magnetic Bead
Sample Type
Cell Culture Supernatants
Scale
Mini
Final Product Type
Genomic DNA
High-throughput Compatibility
High-throughput Compatible
No. of Reactions
100 Reactions
Prep Scale
100 μL of mammalian cell culture samples
Product Line
PrepSEQ™
Target Organism Class
Mycoplasma spp.
Test Time
2 hr.

Contents & Storage

Includes buffers, magnetic beads and enzymes for DNA extraction for Mycoplasma DNA extraction

Figures