Megaplex™ RT Primers, Rodent Pool B v3.0

Designed to streamline experimental workflows, Megaplex™ RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis詳細を見る

製品番号（カタログ番号）	数量
4444292	50 reactions

製品番号（カタログ番号） 4444292

価格（JPY）

125,900

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50 reactions

Designed to streamline experimental workflows, Megaplex™ RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis on a TaqMan® Array MicroRNA Card. Rodent Pool B v2.0 contains RT primers for 306 and 135 unique microRNAs for mouse and rat respectively plus 4 species specific controls. The microRNA targets represented in this pool tend be less well characterized, more narrowly expressed and⁄or expressed at a lower level when compared to Rodent Pool A. The pool can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.

研究用途にのみご使用ください。診断目的には使用できません。

反応数50反応

プライマーRT Primers

製品ラインMegaplex, TaqMan

製品タイプRT Primer

数量50 reactions

出荷条件Room Temperature

種Rat, Mouse

対応可能対象50 Reactions

使用対象（アプリケーション）RT-PCR

GC-Rich PCR Performance低

PCR法2ステップRT-qPCR

Unit SizeEach

組成および保存条件

Store at -20°C.

よくあるご質問（FAQ）

Can Ct's greater than a cut-off be considered valid results?

Yes they can. However, it is important to recognize the true linearity and detection limits of your assay: Ct values above the cut-off can indicate non-specific amplification, unless your NTC is a true- no-target control, and you have run a statistically significant number of replicates. Any results with Ct above the recommended cut-off need to be validated with individual assays on plates.

What is a good Ct cut-off for the TaqMan MicroRNA Array Cards and TaqMan Advanced miRNA Array Cards? In other words, beyond what Ct should I not trust the data?

The typical Ct cut-off on TaqMan Array Cards is 32, which is equivalent to Ct 35 on a plate (10 µl reaction). Previous studies show that if you use pre-amplification, a Ct cut-off of 29 or 30 can be used to reduce numbers of false positives (see Technical Note Optimized protocols for human or rodent microRNA profiling with precious samples). To ensure that you have selected a correct cut-off, you should run replicates of the same sample and use Ct cut-off before you see an increase in the Standard Deviation.

Why does the negative control well show amplification when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents or the cDNA template are contaminated. Please follow established PCR laboratory best practices.

Why do I have poor reproducibility across technical replicates when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents were not adequately mixed. Ensure that all samples and reagents are mixed well.

When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why are the Ct values for the endogenous controls highly variable across the sample set?

Highly variable values for endogenous controls is most likely due to biological variation. We recommend that you use an alternative endogenous control, such as a non-variable miRNA.

View all Megaplex™ RT Primers, Rodent Pool B v3.0 FAQs