Applied Biosystems™

TaqMan™ Array Human MicroRNA B Cards v3.0

The TaqMan™ Array Human MicroRNA B Card v3.0 contains 384 TaqMan™ MicroRNA Assays enabling accurate quantitation of 377 human microRNAs in as little as 5 hours.

Catalog Number	Quantity
4444910	4 pack

Catalog number 4444910

Price (USD)

3,105.00

Quantity:

4 pack

Price (USD)

3,105.00

The TaqMan™ Array Human MicroRNA B Card v3.0 contains 384 TaqMan™ MicroRNA Assays enabling accurate quantitation of 377 human microRNAs in as little as 5 hours. Three TaqMan MicroRNA Assay endogenous controls are included to aid in data normalization and one TaqMan™ MicroRNA Assay not related to human is included as a negative control. Details on the included assays is available in the Target List file. Purchase of the Megaplex™ RT Primers, Human Pool B v3.0 is required to run this array. An additional preamplification step is enabled by Megaplex™ PreAmp Primers, Human Pool B v3.0 for situations where sensitivity is of the utmost importance and/or sample is limiting.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment)AB 7900 HT 384-Well System

FormDried Down

Label or DyeFAM

Product LineMegaplex

Quantity4 pack

Reaction SpeedStandard

Research CategoryGene Regulation, Epigenetics

TypeArray Human MicroRNA B Card

Format384-well Microfluidic Card

SpeciesHuman

Unit SizeEach

Contents & Storage

Frequently asked questions (FAQs)

Why does the negative control well show amplification when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents or the cDNA template are contaminated. Please follow established PCR laboratory best practices.

Why do I have poor reproducibility across technical replicates when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents were not adequately mixed. Ensure that all samples and reagents are mixed well.

When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why are the Ct values for the endogenous controls highly variable across the sample set?

Highly variable values for endogenous controls is most likely due to biological variation. We recommend that you use an alternative endogenous control, such as a non-variable miRNA.

Why is the Ct value for the no-template control (NTC) <35 for some TaqMan microRNA Assays?

The Ct value for the NTC can be <35 for the following reasons:
- There are non-specific interactions between primers. For NTC information on a specific assay, see the Megaplex Assay Performance File.
- The cDNA template is contaminated. Please ollow established PCR good laboratory practices to prevent contamination.
- The preamplification product was not diluted properly before real-time PCR. Ensure that you dilute the preamplification product according the procedure in the user guide.

Why can I not detect any miRNA in my sample using the TaqMan MicroRNA Assay?

Most likely, the threshold is set too high to detect miRNA in samples with low levels of expression. We recommend adjusting the threshold (Ct) or NOAMP flag threshold (Crt) to an appropriate level.

View all TaqMan™ Array Human MicroRNA B Cards v3.0 FAQs