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Applied Biosystems™

TaqMan™ Gene Expression Assay, VIC primer-limited

Green features
Catalog number: 4448485
Applied Biosystems™

TaqMan™ Gene Expression Assay, VIC primer-limited

Green features
Catalog number: 4448485
Catalog Number
4448485
Unit Size
M (750 reactions), made to order
Price (USD)
546.00
Your Price:
Catalog Number
Unit Size
S (360 reactions), made to order
Price (USD)
328.00
Your Price:
Catalog Number
Unit Size
L (2900 reactions), made to order
Price (USD)
1,226.00
Your Price:
Catalog NumberUnit SizePrice (USD)
4448485M (750 reactions), made to order
546.00
Your Price:
4448484S (360 reactions), made to order
328.00
Your Price:
4448486L (2900 reactions), made to order
1,226.00
Your Price:
Product Overview
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Applied Biosystems TaqMan Gene Expression Assays, VIC primer-limited, are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (VIC) on the 5’ end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3’ end. These assays are primer-limited and ideal for multiplex reactions looking at two different gene targets in the same qPCR well.

Features include:
Easy to use—just add cDNA and master mix and run the qPCR—no melt curves required
Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
Sensitive—TaqMan assays are ideal for measuring low levels of expression or low-abundance targets
Accurate—identify small fold-changes with high accuracy of quantitation
Extensive content—over 1.8 million predesigned assays available for over 25 different species
Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for gene variants with high sequence homology
Ideal for multiplexing—combine one FAM-labeled and one VIC-labeled assay to create a duplex assay for two different gene targets in the same qPCR well
Ideal for control assays—use a VIC primer-limited assay for high-expressing endogenous control genes

Approximate ship time
Made to order: 4–6 days in North America, 6–10 days in Europe

TaqMan Gene Expression assays are the gold standard in real-time PCR gene expression studies, built on more than 20 years of experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed. Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs. TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents. All of our predesigned TaqMan Gene Expression assays are covered by the TaqMan Assays qPCR Guarantee.*

Recommended master mix: TaqMan Fast Advanced Master Mix

*Subject to terms and conditions. For complete details, go to www.thermofisher.com/taqmanguarantee.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

CTA Type
Magellan_PDP_OrderNow
Concentration
60X
Detection Method
Fluorescence
Fidelity (vs. Taq)
60 X
Format
Tube
GC-Rich PCR Performance
High
PCR Method
qPCR
Reaction Speed
Fast
Green Features
Sustainable packaging
Product Line
TaqMan™
Product Type
Gene Expression Assay
Shipping Condition
Room Temperature

Contents & Storage

1 tube containing a 20X (S and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.

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