MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit, compatible with PAXgene® Blood RNA Tubes

Catalog number: 4451894

Ambion™  Related applications: RNA Extraction

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Description

The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit purifies total RNA (including miRNA) from PAXgene® Blood RNA Tubes using proven magnetic bead-based technology. A TURBO DNase™ step efficiently eliminates contaminating gDNA ensuring the highest purity RNA possible suitable for real-time RT-PCR and microarray analysis.

• Flexible purification format—use microfuge tubes, manual 96-well plates, or open-format automated 96-well plates from the same kit
• Simple magnetic bead workflow—no spin columns
• Total RNA without RNase activity, contaminating gDNA, or RT-PCR inhibitors

Maximize Total RNA Yields From Blood Samples
The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit utilizes proven magnetic bead-based technology to purify maximal yields of total RNA from PAXgene® Blood RNA Tubes. The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can recover 3-18 µg from a single PAXgene® Blood RNA tube. A TURBO DNase™ step efficiently eliminates contaminating gDNA that can interfere with downstream applications and ensures the highest purity RNA possible.

The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit performs exceptionally well at purifying miRNA with total RNA from PAXgene® Blood RNA Tubes without having to employ a separate miRNA protocol. One protocol does it all.

The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can also detect miRNA from PAXgene® Blood RNA tubes as shown by TaqMan® gene expression assays. Users are strongly advised to perform appropriate validation studies against their miRNA targets.

Flexible Purification Formats
The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be processed in low-, medium- or high-throughput formats with superior workflow and handling compared to traditional competitive methods.
• For low-throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be used in individual 1.5mL microfuge tubes with the bead?RNA-complex remaining in a single processing tube until the final elution step.
• For medium-to-high–throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be done in a 96-well processing plate with either a single or multi-channel pipettor to accelerate the purification process.
• For high-throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is compatible with manual and automated 96-well processing. Simply process multiple batches of tubes in series or in parallel, through the up-front centrifugation process, to the convenient stopping point, until enough samples can be loaded onto a 96-well processing plate for the RNA purification steps. The RNA purification process can be handled on either open-format liquid handlers, in conjunction with a 96-well magnetic stand on the deck, or magnetic bead movers like the MagMAX™ Express-96 Magnetic Particle Processor (deep well version only).

Rapid Processing Time
Processing times will vary based on format selected, but twelve PAXgene® Blood RNA tubes can be processed manually with the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit in approximately 2 hours.

An Alternative to Full Purification
For users who perform primarily TaqMan®-based real-time RT-PCR experiments, and do not need to archive RNA⁄cDNA, an alternative to the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR. This kit makes it possible to perform expression analysis directly from PAXgene® Blood RNA tubes without conventional RNA purification.

One-Stop Shopping
MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is compatible with our suite of reverse transcription mixes (SuperScript®), PCR master mixes (both TaqMan® and SYBR® based), and other blood workflow products. Simplify the ordering process and reduce freight cost by getting more from one supplier.

Specifications:
• Yield (Range): 3 - 18µg
• Quality (RIN) ): ≥ 8
• Quality (A260⁄280) ): 1.8 – 2.2
• gDNA (w⁄w) ): <1.56%
• Equipment Needed :
     *Refrigerated centrifuge (4°C) with swing bucket rotor capable of 5,000 x g with
     *50 mL conical tube adapters
     *50ml conical tubes
     *For tube format users: Tabletop microcentrifuge or picocentrifuge
• Number of Preps: 96 preparations (blood tubes)

NOTE: Blood tubes and magnetic stands are sold separately.

NOTE: Blood tubes and magnetic stands are sold separately.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

PAXgene® and PreAnalytiX® are registered trademarks of PreAnalytiX GmbH. No sponsorship, endorsement, or affiliation is implied herein.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Blood
High Throughput Compatibility: Automated Protocols, High Throughput-Compatible, Not High Throughput-Compatible (Manual)
Downstream Application: Real-Time Quantitative PCR (qPCR)
Format: Tube(s), 96-well plate
Product Size: 96 preps
Final Product: Total RNA
Yield (up to): 18 µg
Purification Time: 1.7 hrs
Isolation Technology: Magnetic Bead

Contents & storage

Kit Components
Box 1:
Store at RT
• 1 PAXgene® Resuspension Solution, 18 mL
• Wash Solution 1 Concentrate, 16 mL (Before using the Wash Solution 1 Concentrate, add the correct volume of isopropanol (user-supplied), as indicated on the bottle)
• Wash Solution 2 Concentrate, 2 x 30 mL (Before using the Wash Solution 2 Concentrate, add the correct volume of ethanol (user-supplied), as indicated on the bottle)

• RNA Rebinding Buffer 10 mL
• Elution Buffer 10 mL
• Processing Plate (96-well) 1 plate
• 2.0 mL Tubes 100 tubes

Store at 2 to 8 °C, Do not freeze
• RNA Binding Beads, 2 mL

Box 2:
Store at -15 to -25 °C
• 2 PAXgeneR Proteinase, 0.6 mL
• 10x TURBO™ DNase Buffer, 1 mL
• TURBO™ DNase, 1 mL

Documents

Manuals & protocols