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Applied Biosystems™

PrepSEQ™ 1-2-3 Nucleic Acid Extraction Kit

Catalog number:  4452222

 Related applications:

Contaminant & Impurity QC Testing

| Mammalian Cell Culture

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The PrepSEQ® 1-2-3 Nucleic Acid Extraction Kit uses magnetic particle-based separation
technology to extract DNA from Mycoplasma cells or Mouse Minute Virus
(MMV) isolated from a variety of starting material, such as infected cell cultures
or Mycoplasma liquid cultures.

Using this kit, you prepare lysates of samples before you extract the target DNA.
For samples that contain Mycoplasma, the lysates contain Mycoplasma DNA
from the free organisms in the culture media and from organisms released from
the infected host cells.
WARNING: Reproductive Harm -
For Research Use Only. Not for use in diagnostic procedures.


Sample Type (General): Liquid Samples (e.g. Serum)
Downstream Application: Cell Culture Contamination Detection
Final Product: Bacterial DNA, Genomic DNA, Viral DNA
Isolation Technology: Magnetic Bead
Product Line: PrepSEQ™
Product Size: 100 preps
Sample Type (Specific): Cell Culture Supernatants
Target Organism Class: Mouse Minute Virus (MMV), Mycoplasma spp., Vesivirus

Contents & storage

• PrepSEQ Proteinase K (store at -15 to -25°C)
• RNASE Cocktail (store at -15 to -25°C)
• PrepSEQ Extraction Kit including Lysis Buffer, Proteinase K Buffer, Binding Solution, Wash and Elution Buffers (store at room temperature)
• PrepSEQ Magnetic Beads (store at room temperature)


Manuals & protocols