Nunc™ Immuno TSP Lids - FAQs

Please review the following applications for TSP (Transferable Solid Phase) :
- Reactions such as the ELISA can be performed on the TSP. The pins are coated by submerging in the analyte solution contained in a 96-well plate. Washing and reaction with succeeding antibody or streptavidin conjugates can be performed by transferring the TSP into a washing tray or second 96-well plate filled with the appropriate solution. The TSP is placed into a substrate solution until color is observed and is then removed to ensure a simultaneous start and stop to the enzymatic reaction.
- TSP is available sterile for screening hybridoma cells for the production and secretion of monoclonal antibodies.
- TSP can be used for radioimmunoassays because it can be placed directly on X-ray film and exposed for several hours. Only the tips of the pins should be incubated with the radiolabeled reagent.
- TSP can be used with the OmniTray for performing dot blots and for replicating bacterial clones from a 96-well plate.

The TSP (Transferable Solid Phase) is a disposable 96 pin device on which solid phase reactions can be performed. It is available with a PolySorp and MaxiSorp surface. The pins are coated by submerging in analyte solution contained in a 96-well plate. Washing and reaction with succeeding antibody or streptavidin conjugates can be performed by transferring the TSP into a washing tray. For hybridoma screening, the TSP is available in a sterile version. The TSP can be used for a simultaneous detection of two different molecules in the same solution.
Advantages include:
- identical reaction times on all pins
- no need for plate washer/dispenser
- allows for a second solid phase reaction to be conducted in a single 96-well plate.