Phospho-FAK (Ser910) Polyclonal Antibody - FAQs

How does the phosphorylation of FAK affect its function?

Focal adhesion kinase (FAK) can be phosphorylated at different sites, this affects its functions as follows:
FAK [pSer722]: Activated in response to cell adhesion; thought to function in opposition to multiple tyrosine phosphorylation events on FAK.
FAK [pSer732]: This is the site phosphorylated by CDK5. The functional importance is unknown but may play a role in cell cycle regulation in the brain, in particular.
FAK [pSer843]: Activated via cell adhesion, and during mitosis it is thought to function in opposition to multiple tyrosine phosphorylation events on FAK.
FAK [pSer910]: Activated via cell adhesion, and during mitosis it is thought to function in opposition to multiple tyrosine phosphorylation events on FAK.
FAK [pTyr397]: Autophosphorylation site (initial activation step) allows Src & p85 subunit of PI3-kinase to bind, and activates cell migration and invasion.
FAK [pTyr407]: Site is activated by TGFbeta and the Epithelial-Mesenchyme Transition (EMT); unlike other sites on FAK, phosphorylation of Tyr407 is EGFR independent.
FAK [pTyr576]: This site is phosphorylated by Src, and is located in the kinase activation loop (YY 576/577 motif); these sites appear to be differentially regulated by stimuli.
FAK [pTyr577]: This site is phosphorylated by Src, and is located in kinase activation loop (YY 576/577 motif), these sites appear to be differentially regulated by stimuli.
FAK [pY861]: This is also a Src site (major), it promotes binding to the cytoplasmic tail of integrins.
FAK [pY925]: This is a Src site (weak), it is GRB2 binding site allowing activation of the Ras/Raf/MEK signaling pathway.

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